Centrifuge-Free Clarification of Large-Volume Bacterial Cultures Using Sartoclear Dynamics® Lab

Authors: Fabio Antenucci, Toloe Allahghadry, Bradley Joseph Whitehead, Anders Miki Bojesen  |  Last updated: Aug 25, 2022

Overview

Clarification of bacterial cultures is often performed by centrifugation and subsequent dead-end microfiltration, a cumbersome and time-consuming process, particularly when large volumes of cultures need to be treated. One example is the production of vaccine prototypes based on outer membrane vesicles (OMVs), a type of bacterial vesicle which has been shown to possess great immunogenic potential and has been a central focus of the research conducted in our group.

In this study we describe the optimization of a novel Sartoclear Dynamics® Lab protocol for the production of large volumes of cell-free bacterial isolates without employing any centrifugation step. 


  • Document type: Application Note
  • Page count: 8
  • Read time: 12 Minutes


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Key Takeaways

  • Sartoclear Dynamics® Lab can be effectively employed for the clarification of large volumes of high density bacterial cultures without hindering yield and quality of OMVs
  • The employment of Sartoclear Dynamics® Lab offers improved sample handling and a greater potential for scalability than traditional centrifuge-based approaches

This Resource is Designed for:

  • Lab researchers

Applications Supported:

  • Clarification of E.coli bacterial cultures

Application Note Preview

Figure 1. Experimental design of this study.

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