Lab Filtration: Centrifuge-Free Clarification of Large-Volume Bacterial Cultures Using Sartoclear Dynamics® Lab
Authors: Fabio Antenucci, Toloe Allahghadry, Bradley Joseph Whitehead, Anders Miki Bojesen | Last updated: Aug 25, 2022
Clarification of bacterial cultures is often performed by centrifugation and subsequent dead-end microfiltration. This is a cumbersome and time-consuming process, particularly when large volumes of cultures need to be treated. One example is the production of vaccine prototypes based on outer membrane vesicles (OMVs), a type of bacterial vesicle which has been shown to possess great immunogenic potential and has been a central focus of the research conducted in our group.
In this study, we describe the optimization of a novel Sartoclear Dynamics® Lab protocol for production of large volumes of cell-free bacterial isolates without employing any centrifugation step.
- Document type: Application Note
- Page count: 8
- Read time: 12 Minutes
- Sartoclear Dynamics® Lab can be effectively employed for clarification of large volumes of high-density bacterial cultures without hindering yield and quality of OMVs
- The employment of Sartoclear Dynamics® Lab offers improved sample handling and a greater potential for scalability than traditional centrifuge-based approaches
This Resource is Designed for:
- Lab researchers
- Clarification of E.coli bacterial cultures