Water Makes the Difference - Ultrapure Water Improves Results in 2D Gel Electrophoresis
Authors: Anja Griebel, Christian Obermaier ,Georg Wrettos & Elmar Herbig | Last updated: March 2021
Overview
Are there differences in quality between stored and freshly prepared ultrapure water? This article discusses the use of ultrapure water in gel electrophoresis and provides recommendations to produce ultrapure water.
The quality of 2D gel electrophoresis results is affected by many factors, with the type of electrophoresis system used and the purity of the chemicals employed among the most decisive. While the advantages of using horizontal high-performance electrophoresis (HPE) over conventional vertical systems have already been shown, this study examines the effect of water quality on silver staining.
- Document type: Application Note
- Page count: 7
- Read time: 8 minutes
Key Takeaways
- How the quality of ultrapure water affects 2D gel electrophoresis results
- The advantages of horizontal high-performance electrophoresis (HPE) over conventional vertical systems
- How silver staining is affected by water quality (freshly produced ultrapure water vs. stored water in a flask)
- How to effectively produce ultrapure water with Arium® Pro VF
This Resource is Designed for:
- Lab Technicians
- Lab Managers
- Post Doc Students
- Chemists and Analytical Scientists
- Pharmaceutical Researchers
- Quality Control or Validation Lab
- Biologists and Life Scientists
- Academic Researchers
Applications Supported:
- Life Science Research
- Water Purification
- Two-Dimensional Gel Electrophoresis
- 2D PAGE
- Protein Separation
- Silver Staining
- Ultrapure ASTM Type 1 Water Quality
- Arium® Pro VF Ultrapure Water Systems
