On-Demand Webinar: From iPSCs to Organoids: Streamlining Hepatic Differentiation
Duration: 27 minutes
Overview
The value of stem cells in laboratory and clinical research has been clearly demonstrated through numerous studies, showcasing their potential as models for a wide range of diseases and therapeutic developments. The inherent flexibility of stem cells allows for the development of various tissue types from a single, highly proliferative base cell type. Differentiation of induced pluripotent stem cells (iPSCs) into somatic tissues requires specific conditions tailored to the desired tissue type.
In this webinar, we describe the process of differentiating human iPSCs into hepatocytes using three distinct media types, each supplemented with specific growth factors and cytokines at key stages of differentiation. This method provides a relatively straightforward approach to producing mature hepatic cells, enabling the generation of human hepatic organoids from these source cells. This demonstrates a robust method for unlimited organoid development.
In this on-demand webinar, we present a simple, standardized, and robust workflow that utilizes Research Use Only (RUO) Growth Factors and Cytokines, combined with high-throughput screening cytometry for phenotypic characterization and live-cell analysis for monitoring cell growth.
This approach simplifies the generation of functional hepatic cells and organoids from iPSCs, facilitating drug discovery, development, and toxicity studies.
Key Takeaways
- How to differentiate iPSCs into Hepatic lineage cells and organoids
- Best practices for monitoring differentiation, while confirming lineage and function
- Dissociation of complex 3D structures into healthy single cells for analysis
- How Sartorius products can aid in the simplification and development of robust protocols for organoid production
Webinar Speaker
Daryl Cole, Ph.D.
Scientist
Application Development
Sartorius
Daryl Cole earned a PhD in Molecular Oncology from King’s College London in 2008 and joined Sartorius in 2021 as a Scientist in the Bio Applications Team, working with the Incucyte®, Octet®, iQue®, and CellCelector but with a focus on CellCelector applications development. He has over 15 years of experience in a range of scientific fields from cancer research to organoid and stem cell development.