Optimization of Human iPSC-derived Neuronal Mono- and Co-cultures with Microglia
Authors: Jasmine Trigg, Daryl Cole, Natasha Lewis, John Rauch, and Nicola Bevan
Models derived from human induced pluripotent stem cells (iPSCs) are becoming more prevalent for in vitro research on the human central nervous system. These models facilitate the creation of specific types of neurons and supporting cells, offering a system that more closely mirrors human biology for the study of development and disease.
The role of growth factors and cytokines is crucial in these models for promoting cell growth, differentiation, and survival. However, their analysis is often complex due to variables such as undisclosed ingredients in commercial media and the cells' sensitivity to manipulation.
To effectively utilize these sophisticated models, it is essential to establish stable culture conditions and employ technological methods that allow for the non-destructive optimization, visualization, and dynamic measurement of these intricate systems.
Overview
In this application note, we explore the effectiveness of Sartorius Research Use Only (RUO) Growth Factors and Cytokines in consistently sustaining iPSC-derived neuronal and microglial models, both in normal and pathological conditions.
Additionally, we illustrate how the Incucyte® Live-Cell Analysis System aids in their assessment by providing non-invasive, ongoing observation of culture vitality, morphology, marker expression, and cellular functionality.
Document type: Application Note
Page count: 13
Read time: 23 minutes
Last updated: April 2024
Download the application note and learn:
- A method for monitoring and investigating iPSC-derived neuronal and microglia cultures in healthy and diseased states
- How to leverage growth factors and cytokines for robust maintenance of iPSC-derived neuronal and microglial models in
- How to utilize live-cell analysis to facilitate characterization through non-invasive, continuous monitoring inside the incubator
Preview of Application Note