Optimization of human iPSC-derived neuronal mono- and co-cultures with microglia

Authors: Jasmine Trigg, Daryl Cole, Natasha Lewis, John Rauch, and Nicola Bevan

Resource overview

Human iPSC-derived models are increasingly used to study the central nervous system, enabling the generation of specific neurons and supporting cells that closely mimic human biology. Growth factors and cytokines are critical for cell growth, differentiation, and survival, but analysis is complex due to media variability and cell sensitivity. Stable culture conditions and non-destructive monitoring are essential. This application note highlights Sartorius RUO Growth Factors and Cytokines for sustaining iPSC-derived neuronal and microglial models and shows how the Incucyte® Live-Cell Analysis System enables continuous, non-invasive observation of culture health, morphology, marker expression, and functionality.
 

Download the application note and learn:

  1. A method for monitoring and investigating iPSC-derived neuronal and microglia cultures in healthy and diseased states
  2. How to leverage growth factors and cytokines for robust maintenance of iPSC-derived neuronal and microglial models
  3. How to utilize live-cell analysis to facilitate characterization through non-invasive, continuous monitoring inside the incubator
     

Resource details:

  • Document type: Application note
  • Page count: 13
  • Read time: 23 minutes

Request a Quote, Demo, or More Information

What other areas are you interested in? (select all that apply)

Access the app note