Preparing 3D cell cultures
Authors: Sartorius and Biotechniques | Last updated: December 2024
Resources overview
Preparing reproducible 3D cell cultures can be challenging. Variability in handling and pipetting can affect the consistency of tumor spheroids, organoids and other 3D cell culture models. These resources help researchers address common challenges in 3D cell culture preparation with practical guidance on reproducibility and pipetting.
Explore practical resources for researchers working with 3D cell cultures, including expert guidance on preparation, factors affecting reproducibility, common pipetting challenges and techniques to improve consistency.
Resources
Podcast: Preparing 3D cell cultures
In this podcast, we explore preparing 3D cell cultures with Sandra Söderholm, Application Development Specialist, and Maria Voutilainen, Product Manager from Sartorius. Sandra and Maria introduce 3D cell cultures and how to prepare them, and then delve into a foundational technique that is often overlooked: pipetting. We discuss pipetting techniques, common mistakes and their pipetting dos and don’ts.
Video: Reproducibility of 3D cell cultures
Although the use of 3D cell cultures is growing, reproducibility remains an issue in the field. One reason for this is that preparing 3D cell cultures can be technically challenging, so the cultures are prone to variability. In this video we explore common factors that impact reproducibility and how to overcome them.
Infographic: Tips and tricks for pipetting 3D cell cultures
Although the use of 3D cell cultures has grown rapidly in recent years, they can be challenging to prepare. In this infographic, we’re going back to basics and looking at a foundational technique for many lab-based researchers: pipetting. Explore common challenges faced when pipetting 3D cell cultures and some tips to overcome them.
Application note: Best practices for pipetting 3D cell culture hydrogels
Inconsistent pipetting can introduce unwanted variation into 3D cell culture experiments. Learn practical techniques to improve consistency in sample preparation and mixing.