Microbial Enumeration Solutions | Microsart® & Biosart®

Ensure safety, speed, and compliance in microbial testing with ready-to-use, single-use membrane filtration systems designed for bioburden testing and microbial monitoring across pharmaceutical, water, food, beverage, and cosmetic applications.

Both Microsart® @Filter and Biosart® 100 Monitors are preassembled and gamma-irradiated for immediate use, helping eliminate cleaning, sterilization, and cross-contamination risks. With certified 0.2 µm and 0.45 µm membranes, including high-flow variants, these solutions support faster filtration, consistent microbial recovery, and improved operator safety—no flame sterilization or autoclaves required.

 

Key Benefits at a Glance

  • Sterile and Ready to Use - Preassembled and gamma-irradiated—no cleaning, decontamination, or setup time required.
  • Efficient, Contamination-Free Operation Click-fit (Microsart® Filter) or integrated petri dish design (Biosart® 100 Monitors) eliminates membrane handling.
  • Faster Filtration with High Recovery High-flow membrane options speed up filtration by up to 30%, ideal for turbid samples.
  • Touch-Free Membrane Transfer Microsart® Filter enables direct membrane transfer when paired with Microsart® Media.
  • Operator-Friendly Design Minimizes repetitive strain, avoids flame sterilization, and supports high-throughput workflows.
  • Supports a Range of Applications Validated for microbial recovery in pharma QC, water, beverages, food, and cosmetics.

Filtration Units

Microsart® @filter

Sterile, validated, ready-to-use filtration units designed for bioburden testing in pharmaceutical quality control. Touch free membrane transfer.

Biosart monitors

Biosart® 100 Monitors

Sterile, ready-to-use filtration monitors for applied industries. No membrane transfer-filtration & cultivation in one. 

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Microsart® @Filter 

Microsart® @Filter

Microsart® @Filter is a sterile, ready-to-use filtration unit designed for bioburden testing and microbial enumeration of bacteria, yeasts, and molds—even at low concentrations. Built for membrane filtration workflows in regulated environments, this single-use solution helps reduce contamination risks, preserve sample integrity, and increase lab efficiency.

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Key Benefits at a Glance

  • No Cleaning or Sterilization Pre-assembled and gamma-irradiated, ready for immediate use on stainless-steel manifolds.
  • Reliable, Contamination-Free Operation — Secure click-fit closure enables safe funnel removal; touch-free membrane transfer when paired with Microsart® @Media.

  • Accurate Sample Handling — Graduated 100 mL and 250 mL volume markings with transparent housing support precise rinsing of inhibitors like antibiotics and preservatives.
  • Flexible Membrane Choices — Available with plain or gridded CN, PVDF, RC, or PC membranes, compatible with aqueous, alcoholic, and organic solutions.

Key Features and Benefits

Image of a scientist using Microsart @filters and e.jet pump
Image of a scientist using Microsart @filters and e.jet pump
Microsart® @filter 250 product image - in packaging and out of packaging
Touch-Free Membrane Transfer

When combining the usage of the Microsart®@Filter with the Microsart@media: the patented adhesive lid of the media plate guarantee a touch-free membrane transfer from filter base to the agar plate minimize the risk of secondary contamination.

Easy Membrane transfer

For use with standard 60 or 90mm agar media plates, an easy removal of the membrane is possible with the gabs on the base

Flexibility in Packaging

3 different packaging versions available – stackable in bags, on trays or single sterile packed

Adaptable to your application

Different membrane filter versions (Cellulose Mixed Ester, PVDF, Polycarbonate and Regenerated Cellulose), Pore sizes (0.2µm, 0.45µm and 0.65µm), membrane colors (Mixed Cellulose Ester: white, black and green) and funnel volumes (100 mL and 250 mL) available – depending on your application

Tool-free Funnel Removal

The bayonet closure allows easy adjustments onto - and removal from - the stainless-steel manifold

Available Microsart® @Filter Variants and Combinations

Membrane MaterialPore SizesMembrane ColorFunnel VolumesPackaging version
Cellulose Nitrate (Mixed Cellulose Ester)0.2µm, 0.45µm, 0.45µm Highflow, 0.65µmwhite w/o grid, white with black grid, grey with white grid, green with dark-green grid100 mL, 250 mLbag, tray, single sterile
PVDF0.45 µmwhite - plane100 mLbag, tray, single sterile
Regenerated Cellulose (RC)0.45 µmwhite - plane100 mLbag, tray 
Polycarbonate (PC)0.2 µm, 0.45 µm white - plane100 mL, 250 mLbag

Lid Inclusion: Bag version comes without lid (can be ordered separately). Tray and single sterile packaging comes with lid.

Biosart® 100

Biosart monitors

Biosart® 100

Biosart® 100 Monitors are ready-to-use, sterile filtration units designed for reliable microbial testing in water, food, and beverage quality control. Stable materials support their optional reusability via autoclaving. Purpose-built for applied industry workflows, these single-use systems simplify colony enumeration of bacteria, yeasts, and molds—minimizing handling steps and reducing contamination risks.

Unlike traditional methods, Biosart® 100 Monitors coupled with Biosart® 100 Media eliminate the need to transfer the membrane to a separate agar plate. Once the filtration of the sample and Biosart® 100 Medium is complete, the funnel/cylinder detaches easily and doubles as a Petri dish for incubation, creating a fully self-contained solution from filtration through to colony growth and analysis.

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Key Benefits at a Glance

  • Ready-to-Use & Sterile — Preassembled and pre-sterilized units for immediate use, eliminating the need for cleaning or autoclaving.
  • Reliable Microbial Testing — Designed for water, food, and beverage quality control, ensuring dependable results.
  • Simplified Workflow: Single-use systems minimize handling steps and reduce contamination risk.
  • Self-Contained Solution — Funnel/cylinder detaches to double as a Petri dish, eliminating membrane transfer to separate agar plates.

  • Easy Integration — Compatible with standard vacuum manifolds for seamless filtration to incubation.
  • Versatile Membrane Options — Available in various pore sizes and color-coded membranes for optimized colony visibility and accurate counting.
  • Superior Microbial Recovery — Cellulose nitrate filters ensure high recovery rates for bacteria, yeasts, and molds.

Key Features and Benefits

Image of a scientist using Microsart @filters and e.jet pump
No Risk of Contamination

The Biosart Monitor doubles as a filtration unit and petri-dish

Easy Colony Counting

Membranes available in multiple pore sizes & white, grey/black and green to visualize colonies of different colours

Multiple Media types

  Chose from multiple different pre-sterilized media in ampoules

  Fully compliant with ISO standards, the American standards for water and foods, mineral water regulations and other guidelines of the food and beverage industries

Reliable Vacuum Performance

Adaptors available for use with other filtration manifolds/devices

Biosart® 100 Monitor Variants and Combinations

Item #

Pieces

Packaging

Description

 
ACK48Individually PackedEach monitor is individually sterile-packed and then placed into a carton. Sartorius Biosart 100 Monitors open product box and examples of contents.
K48Bags/Tray4 sterile-packed trays, each containing 12 monitors, are placed into one carton. Sartorius Biosart 100 Monitors open product box and examples of contents.
V--K48Bulk48 sterile monitors (not individually sterile-packed) are packed in a carton and sealed with an overfoil. Sartorius Biosart 100 Monitors open product box and examples of contents.

 

How to use 

Biosart® 100 Monitors and Media

Learn how to use and convert Biosart®️ 100 Monitors to ensure accurate and contamination-free microbial testing in water and beverages.

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Using Microsart® @filters and @media

Learn how to perform a touch-free membrane transfer step-by-step to ensure safe and contamination-free handling.

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Resources

Featured Microbial Enumeration Solutions

Application Note

Recovery of Burkholderia cepacia Complex Species

Using Microsart® @filter

Robust Microbial Recovery After Filtration of Disinfectants Using Microsart® @filter PVDF
Application Note

Robust Microbial Recovery Using Microsart® @filter PVDF

Learn how the Microsart®@filter PVDF is a robust solution that ensures effective recovery of microorganisms after filtration of various disinfectants.

microsart
Application Note

Filtration of Viscous Samples for Microbiological Quality Control

Optimizing microbial enumeration in viscous substances is critical. This study explores how temperature & dilution with IPM impacts, using Microsa...

Application Note

Saving Time With Disposable Filtration Units Versus Reusable Filtratio...

The study focuses on typical applications within the beverage market segment. 

Microbial Enumeration Solutions Documentation & Literature

Application note & guide

Application Guide

Microbiology Wine Preventive Care Monitoring Testing Methods - Applica...

PDF 11.7 MB
Application Note

Recovery of Burkholderia cepacia Complex Species

Using Microsart® @filter

Lab technician decontaminating a biosafety cabinet
Application Note

Microbial Enumeration of Disinfectants

Learn how the Microsart®@filter PVDF is a robust solution that ensures effective recovery of microorganisms after filtration of various disinfectants.

microsart
Application Note

Filtration of Viscous Samples for Microbiological Quality Control

Optimizing microbial enumeration in viscous substances is critical. This study explores how temperature & dilution with IPM impacts, using Microsa...

Application Note

Saving Time With Disposable Filtration Units Versus Reusable Filtratio...

The study focuses on typical applications within the beverage market segment. 

Datasheet

Datasheet

Microsart® @filter 100 | 250 — Sterile single-use filter units featuri...

PDF 246.4 KB
Biosart monitors
Datasheet

Biosart® 100 Monitors

Ready-To-Use Sterile Filtration Units for Germ Count Determination

Datasheet

Biosart® 100 Nutrient Media — Microbiological Nutrient Media for Filtr...

PDF 139.0 KB

User guide

User Guide

Touch-Free Membrane Transfer Using Microsart @filter and @media

PDF 938.5 KB

Frequently Asked Questions

Microsart® @Filter is a sterile filtration unit (funnel, membrane filter and filter support) ideal for workflows that require transferring a membrane onto agar plates. 
Biosart® 100 monitors combine funnel, membrane filter, cellulose pad, and sterile filter support in a single filtration unit. After adding the liquid medium from the ampoule (Biosart medium), the filtration unit is directly converted into a Petri dish—no membrane transfer is required.

Both product lines offer 0.2 µm and 0.45 µm membranes. High-flow variants are available for faster filtration of turbid samples.

Microsart® @Filter is available in 100 mL and 250 mL funnel options. Biosart® 100 Monitors are designed for typical sample volumes used in QC workflows.

Microsart® @Filter pairs with Microsart® stainless-steel manifolds, Microsart e.jet liquid transfer pump and Microsart® @Media plates or alternatively standard 60 or 90mm agar plates. Biosart® 100 Monitors work with Microsart® stainless-steel manifolds (version Biosart 100), the Microsart e.jet liquid transfer pump and Biosart Media in Ampoules.

Yes. Both systems are designed to support GMP and quality standards in pharmaceutical and applied microbiology labs.

Microsart® @Filter is available in different membrane types:

  • Gridded Cellulose Nitrate (Mixed Cellulose Ester) membranes for filtration of aqueous solutions
  • Plane Cellulose Nitrate (Mixed Cellulose Ester) membranes for Automated Colony Counting Applications
  • PVDF membrane for filtration of alcoholic and organic solutions
  • Regenerated Cellulose for filtration of solvents and acidic industrial process waters (pH range 3 – 12)
  • PC membranes for retention of Microorganisms for further analysis (e.g. where you need to flush the microbes from the membrane, usage for Rapid Testing Methods)

The system allows thorough rinsing of inhibitors like antibiotics and preservatives, minimizing interference in microbial recovery.

Choose between 100 mL and 250 mL options with clear volume markings.
100 mL funnel has marks at 20, 50 and 100 mL. 250 mL funnel has marks at 50, 100, 200 and 250 mL.

It eliminates sterilization and handling steps, reduce handling time and operator fatigue, ensures reliable recovery, even in large or inhibitor-containing samples.

When paired with Microsart® @Media, membranes can be moved touch-free using an integrated adhesive lid.

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