Accurate High-Throughput Surface Plasmon Resonance for Rapid Biologic Discovery

The early identification of a compound’s kinetics, affinity and competitive properties is crucial for making accurate decisions in the initial stages of the drug discovery process. This important phase can often be exacerbated by the requirement to investigate large compound libraries to identify molecules with the most advantageous biological properties.

Accordingly, the entire Surface Plasmon Resonance (SPR) range by Sartorius has been designed with one clear focus – to allow researchers to generate the most accurate data possible in the shortest timeframe imaginable. 

This is made possible by the use of patented tools exclusive to the Octet® SPR range, which include:

  • OneStep® Gradient Injections: Eliminate the need for multiple analyte concentrations and significantly reduce assay development time to generate kinetics for up to 768 molecules in less than 24 hours
  • OneStep® Two Component Injections: Determine accurate kinetics across multiple conditions and in the presence or absence of cofactors
  • NeXtStep™ Gradient Injections: Highly accurate competition assays to examine the behavior of an analyte in the presence of a competitor molecule

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Boost your research and commercialization goals by combining BLI and SPR at an attractive promotional price. 

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Discover Octet® SPR with unique patented injection modes

Explore the Octet® Surface Plasmon Resonance Range

Octet® SF3 SPR

Octet® SF3 SPR 

Generate high-quality kinetics and affinity data in a fraction of the time compared to standard multi-cycle kinetics using the Octet® SF3.

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Octet CLD Sensor Chip

Octet® SPR Sensor Chips and Consumables

Sensor chip selection is a critical feature of every SPR assay, with each Octet® SPR sensor chip ensuring accurate and reproducible results.

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Octet SF3 SPR system

Octet® SPR Service Contracts

Octet® SPR services detect potential issues before they develop into future problems to help maximize instrument productivity.

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Features of the Sartorius Octet® SF3

Octet® SF3 SPR
Octet® SF3 SPR
NeXtStep™ Gradient Injections
Octet SF3 Racks
OneStep® Injection Technology
  • Two standard injection formats: OneStep® Injection Technology and industry standard multi-cycle kinetics (MCK)
  • Eliminate the need to prepare concentration dilution series for each analyte, saving on time, reagents and plate space, and reducing potential errors in assay development
  • Diffuse a single concentration of analyte into a stream of buffer to create an analyte concentration gradient of at least three orders of magnitude 
  • Accurate measurement of binding kinetics and affinity from a single analyte concentration

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NeXtStep™ Gradient Injections
  • Determine the behavior of an analyte in the presence of a competitor molecule from a single injection
  • Assess multiple analytes and competitors in a single assay without the need for the competitor to be present in the running buffer 
  • Clearly determine full binding kinetic profiles, affinity and site-specific competition as a modulation of binding in the presence of a competitor molecule

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High-Throughput Sample Acquisition
  • Generate complete kinetics and affinity for up to 768 samples in a single unattended assay
  • OneStep® Injection Technology: More than 72 hours unattended run time and a unique sample layout allows for high-throughput acquisition and analysis of hundreds of samples in a single run, making the Octet® SF3 suitable for even the most high-throughput library screens

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Octet® Surface Plasmon Resonance Resources

Featured Resource

A compendium for successful BLI and SPR assays
Application Guide

A Compendium for Successful BLI and SPR Assays

Octet® Label-Free Biosensor Analysis

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Octet® SPR Downloads and Guides

Find the latest guides, application notes, white papers and more for the next generation Octet® SF3 Surface Plasmon Resonance (SPR) platform.


Octet SF3 Instrument Brochure

PDF 4.8 MB

Octet® SPR Sensor Chips

PDF 1.3 MB

Octet® SF3 SPR Service Plans

PDF 2.5 MB

Octet SF3 Pulse Injection - Brochure | Sartorius

PDF 1.7 MB
Octet SF3 SPR Flyer cover

Octet® SF3 SPR Instrument

PDF 468.7 KB

Octet® SF3 Consumables

PDF 411.3 KB

Octet® SF3 SPR Maintenance

PDF 601.7 KB
The Next Generation of SPR-Based Interaction Analysis cover

The Next Generation SPR-Based Interaction Analysis

PDF 273.6 KB

Octet® SPR Sensor Chip Selection Guide

PDF 591.8 KB

Best Practice Guide: Octet® SF3 Assay Orientation

PDF 940.3 KB

Best Practice Guide: Minimize Artifacts 

PDF 861.4 KB

Best Practice Guide: Octet® SF3 System Maintenance

PDF 187.3 KB

Best Practice Guide: Minimizing the Effects of Non-Specific Bindi...

PDF 120.0 KB

Best Practice Guide: Double Reference Subtraction

PDF 868.5 KB
Best Practice Guide: Octet SPR Sensor Chip Preparation

Best Practice: Octet® SPR Sensor Chip Preparation

PDF 99.2 KB
Octet SPR Analysis User Guide
User Guide

Octet® SPR Analysis User Guide

PDF 2.5 MB
Octet SPR Discovery Software User Guide
User Guide

Octet® SPR Discovery Software User Guide

PDF 7.6 MB
application note thumbnail
Application Note

Kinetics Determination of High Affinity Molecular Interactions Us...

PDF 212.2 KB
Application Note

Kinetic Curvature: Assessment of Small Molecule Kinetics and Affinity...

PDF 289.9 KB
SPR Fragment based drug discovery
Application Note

Fragment-Based Drug Discovery (FBDD) Using Octet® SPR OneStep® and NeX...

PDF 5.1 MB
OneStep lead characterization of high affinity biologic interactions
Application Note

OneStep® Lead Characterization of High Affinity Biologic Interactions

PDF 2.2 MB
Application Note

Recovery of Sample Using the Octet® SF3 - Application Note | Sartorius

PDF 1.4 MB

Guidelines for OneStep®️ Assay Design

PDF 3.4 MB

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