Preparing 3D Cell Cultures

Overview

Our understanding of the development of organs, the pathogenesis and progression of diseases and the impact of therapeutics on tissues has advanced in recent years with the help of in vitro 3D cell culture models, including single and multicellular tumor spheroids and organoids. Although the use of 3D cell cultures is growing, preparing 3D cell cultures can be technically challenging, so the cultures are prone to variability.

Learn more about preparing 3D cell cultures, including the issue of reproducibility and how to overcome common technical challenges when pipetting.

In this podcast, we explore preparing 3D cell cultures with Sandra Söderholm, Application Development Specialist, and Maria Voutilainen, Product Manager from Sartorius. Sandra and Maria introduce 3D cell cultures and how to prepare them, and then delve into a foundational technique that is often overlooked: pipetting. We discuss pipetting techniques, common mistakes and their pipetting dos and don’ts.

• Document type: Podcast
• Listen time: 11:00 minutes

Although the use of 3D cell cultures is growing, reproducibility remains an issue in the field. One reason for this is that preparing 3D cell cultures can be technically challenging, so the cultures are prone to variability. In this video we explore common factors that impact reproducibility and how to overcome them.

• Document type: Video
• Listen time: 2:40 minutes

Although the use of 3D cell cultures has grown rapidly in recent years, they can be challenging to prepare. In this infographic, we’re going back to basics and looking at a foundational technique for many lab-based researchers: pipetting. Explore common challenges faced when pipetting 3D cell cultures and some tips to overcome them.

• Document type: Infographic
• Page Count: 1

Access the Infographic