Optimize Your iQue®️ Assay With Picus®️ Electronic Pipettes
Authors: Sandra Söderholm, Philip Wiese, Evelyn Marschall | Last updated: September 20, 2022
Overview
The iQue® analysis is extremely fast - it takes just five minutes to measure a complete 96-well plate. The bottleneck tends to be the sample preparation. Assay setup for flow cytometry consists of many pipetting steps - cells are stained with different dyes and, in the case of iQue® kits, beads that bind secreted proteins are also added to the samples. Thus, despite the iQue® TCA kit being a one-wash assay, the sample preparation is rather laborious and can take hours. The protocol includes three 1-hour incubations and multiple pipetting steps.
Learn more about how to optimize your iQue® assay and reduce sample preparation time using Picus® electronic pipettes. Download the application note now!
- Document type: Application Note
- Page count: 10
- Read time: 16 minutes
Key Takeaways
- Picus® electronic pipettes speed up sample preparation.
- Electronic pipettes allow you to use features such as multiple dispensing and automatic mixing steps that are not possible with mechanical pipettes.
- The iQue® Human T Cell Activation (TCA) provides a multiplexed approach to measure T cell activation, proliferation and cytokine release in a single well.
This Resource is Designed for:
- Lab Researchers and Managers
- Scientists
- Clinical Assistant
- Application Specialist
- Engineers
- Chemists
Applications Supported:
- Pipetting
- Flow Cytometry
- T Cell
