Unisart® Nitrocellulose Membranes for Lateral Flow Assays

Our Unisart® nitrocellulose membranes are specifically designed to be the ideal solid phase for all diagnostic lateral flow immunoassays. Every step in the Unisart® membrane manufacturing process has been optimized in order to obtain a reproducible 3D membrane structure with a clean and defect-free membrane surface. The homogenous structure of our membranes ensures a controlled and uniform lateral wicking of samples and reagents across the membrane which leads to improved accuracy and reproducibility of lateral flow test results.

What makes our Unisart® nitrocellulose membranes the best choice for your lateral flow rapid test?

Consistency matters

Reliable capillary speed, high protein binding, homogenous thickness and a smooth and defect-free membrane surface are essential to obtain high quality lateral flow test strips. The exceptionally high inter- and intra-lot consistency of our Unisart® membranes will help you to minimize the variability within lateral flow immunoassays and ensures accurate and precise results. Producing more consistent readouts improves test strip performance and reduces manufacturing costs.

Reliable membrane supply

In-depth know-how in nitrocellulose membrane manufacturing as well as relevant application expertise are driving us to continuously improve our state-of-the-art manufacturing equipment. As a technology leader, we guarantee reliable supply of high quality nitrocellulose membranes and also ensure availability of our Unisart® membranes on large scale.

Quality made in Germany

We focus on quality in every step of our membrane manufacturing which is located in Göttingen, Germany. Our Unisart® membranes are developed, produced and distributed according to a Quality Management System that is certified in compliance with DIN/ISO 9001. All products must pass our precisely defined in-house tests and meet our stringent quality control standards throughout all manufacturing steps.

Complete tracebility

All Unisart® membrane products have a comprehensive and easy to read out labeling which allows convenient retrieval of all data compiled on materials used, production steps and QC tests. Quality assurance certificates guarantee full visual inspection and conformity to specifications for all Unisart® lateral flow membranes.

Unisart® Nitrocellulose Membrane Product Family

Membranes that make a difference

The nitrocellulose membrane is one of the most important components of a lateral flow rapid test and should be selected carefully. Especially the capillary speed of the membrane is very critical for lateral flow strip functionality as it affects sensitivity, specificity, response time and eventually costs of the assay. Each membrane type of our Unisart® nitrocellulose membrane family has a specific range of capillary speed. Our Unisart® CN 95 membrane is perfectly suited for high viscous samples and produces test strips with very short response times. Consequently Unisart® CN 95 is the membrane of choice when a quick response is desirable. In contrast, test strips with our Unisart® CN 180 membrane have the longest response time, but the assays will show the highest sensitivity. According to the application, reagents and manufacturing conditions our Unisart® CN 110, CN 140 and CN 150 membranes provide a different optimum for speed, specificity and sensitivity. Our wide selection of Unisart® membranes allows you to find the one that best meets the requirements of your diagnostic assay.

Unisart® CN 95 backed CN 110 backed CN 140 unbacked CN 140 backed CN 150 
backed
CN 180 
backed
Membrane Material cellulose nitrate cellulose nitrate cellulose nitrate cellulose nitrate cellulose
nitrate
cellulose 
nitrate
Backing 100 µm polyester, clear 100 µm polyester, clear none 100 µm polyester, clear 100 µm 
polyester, clear; 
100 µm 
polyester, white
100 µm polyester, clear
Thickness (µm) 240 - 270 185 - 215 120 - 160 225 - 255 240 - 280 225 - 255
Capillary speed down web, purified water (s/40mm) 65 - 115 90 - 130 90 - 150 95 - 155 90 - 180 135 - 175
Visual appearance All Unisart® membranes offer a white, flat and smooth surface, free of macroscopic defects and foreign matter.
They are all 100% inspected by backlight inspection.
 Wettability Precise amount of anionic surfactant allows for fast wetting
Plastic roll core76.8 mm, 3“
Certificate of analysisprovided with each delivery
Membrane width17—300 mm (+/- 0.5mm)


Membrane wound with yellow interleaving protection paper between the membrane layers.

Unisart® CN 95 
backed
CN 110 
backed
CN 140 
unbacked
CN 140 
backed
CN 150 
backed
CN 180 
backed
Backing 100 µm clear 100 µm clear none 100 µm clear 100 µm clear, 
100 µm white
100 µm clear
Belt side as
application side
no no yes no yes no
Background clearing very fast very fast fast fast fast fast
Time–to-signal fast fast medium medium medium medium
Quick response tests best suitable suitable, 
depending on 
migration distance
suitable, 
depending on 
migration distance
suitable, 
depending on 
migration distance
suitable, 
depending on 
migration distance
suitable, 
depending on 
migration distance
Sensitivity good good very good very good very good, can be enhanced by using 
white backing
excellent
Viscous samples very good good good good good good
Particle loaded samples very good very good good good good good
Backing as barrier 
against card glue
yes yes no, cards need to be selected carefully yes yes yes
Contact dispensing 
equipment
suitable suitable suitable suitable suitable suitable
Non- contact 
dispensing equipment
suitable suitable suitable suitable suitable suitable
Suitable for reel-to-reel manufacturing suitable suitable needs careful 
handling
suitable suitable suitable
Unisart® Membrane Backing Polyester Width (mm) Length (m) Max. Splices Units/ Box Catalog Number
CN 95 backed            
CN 95 100 µm clear 20 50 0 5 rolls 1UN95ER050020WSB
CN 95 100 µm clear 25 50 0 5 rolls 1UN95ER050025WSB
CN 95 100 µm clear 30 50 3 5 rolls 1UN95ER050030—B
CN 95 100 µm clear 20 100 3 1 roll 1UN95ER100020NT
CN 95 100 µm clear 25 100 1 5 rolls 1UN95ER100025KSB
CN 95 100 µm clear 25 100 3 5 rolls 1UN95ER100025NTB
CN 95 100 µm clear 28 100 3 5 rolls 1UN95ER100028NTB
CN 95 100 µm clear 30 100 3 5 rolls 1UN95ER100030—B
CN 95 100 µm clear 35 100 3 5 rolls 1UN95ER100035NTB
CN 95 100 µm clear 40 100 0 1 roll 1UN95ER100040WS
CN 95 100 µm clear 70 100 0 1 roll 1UN95ER100070WS
CN 110 backed            
CN 110 100 µm clear 20 100 3 5 rolls 1UN11ER100020NTB
CN 110 100 µm clear 25 100 3 5 rolls 1UN11ER100025NTB
CN 110 100 µm clear 25 100 3 1 roll 1UN11ER100025NT
CN 140 unbacked            
CN 140 none 200 0.25 0 2 sheets 1UN14AS25020001
CN 140 none 20 50 3 5 rolls 1UN14AR050020—B
CN 140 none 25 50 3 5 rolls 1UN14AR050025—B
CN 140 none 20 100 3 5 rolls 1UN14AR100020NTB
CN 140 none 20 100 0 5 rolls 1UN14AR100020WSB
CN 140 none 25 100 3 5 rolls 1UN14AR100025NTB
CN 140 none 25 100 0 5 rolls 1UN14AR100025WSB
CN 140 none 35 100 3 5 rolls 1UN14AR100035NTB
CN 140 none 40 100 3 1 roll 1UN14AR100040NT
CN 140 none 270 100 3 1 roll 1UN14AR100270NT
CN 140 backed            
CN 140 100 µm clear 20 50 3 5 rolls 1UN14ER050020—B
CN 140 100 µm clear 25 50 3 5 rolls 1UN14ER050025—B
CN 140 100 µm clear 25 50 0 5 rolls 1UN14ER050025WSB
CN 140 100 µm clear 17 100 3 1 roll 1UN14ER100017NT
CN 140 100 µm clear 18 100 3 5 rolls 1UN14ER100018NTB
CN 140 100 µm clear 20 100 3 1 roll 1UN14ER100020NT
CN 140 100 µm clear 20 100 3 5 rolls 1UN14ER100020NTB
CN 140 100 µm clear 25 100 3 1 roll 1UN14ER100025NT
CN 140 100 µm clear 25 100 3 5 rolls 1UN14ER100025NTB
CN 140 100 µm clear 28 100 3 5 rolls 1UN14ER100028NTB
CN 140 100 µm clear 30 100 3 5 rolls 1UN14ER100030—B
CN 140 100 µm clear 35 100 3 5 rolls 1UN14ER100035NTB
CN 140 100 µm clear 40 100 0 1 roll 1UN14ER100040WSB
CN 140 100 µm clear 70 100 0 1 roll 1UN14ER100070
CN 150 backed            
CN 150 100 µm clear 20 50 3 5 rolls 1UN15LR050020NTB
CN 150 100 µm clear 25 50 3 5 rolls 1UN15LR050025NTB
CN 150 100 µm clear 20 100 3 5 rolls 1UN15LR100020NTB
CN 150 100 µm clear 25 100 3 1 roll 1UN15LR100025NT
CN 150 100 µm clear 25 100 3 5 rolls 1UN15LR100025NTB
CN 150 100 µm white 25 100 3 5 rolls 1UN15WR100025NTB
CN 180 backed
CN 180 100 µm clear 20 100 3 5 rolls 1UN18ER100020NTB
CN 180 100 µm clear 25 100 3 5 rolls 1UN18ER100025NTB


Customized dimensions of Unisart® nitrocellulose membranes are also available upon request.

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