Resource overview
This application note presents an evaluation of the Cyclus® Rapid Mycoplasma Workflow for RT-qPCR-based mycoplasma detection in cell culture media and HEK cell matrices. The workflow combines Cyclus® Bead Extraction, the Cyclus® RT-qPCR Mycoplasma kit, and Cyclus® Mycoplasma Standards. The study examines performance at European Pharmacopoeia chapter 2.6.7 (12.2)-relevant levels, including detection in high-density cell samples, extraction efficiency, PCR inhibition, and control performance. Results from two real-time PCR platforms demonstrate consistent detection across the tested matrices and support the workflow’s use in routine quality control and product-specific method validation.
Key takeaways:
- How the Cyclus® Rapid Mycoplasma Workflow detects mycoplasma at European Pharmacopoeia-relevant levels
- How the workflow performs in cell culture media and HEK cell samples
- How extraction efficiency, PCR inhibition, and cross-platform performance support routine quality control and method validation
Resource details:
- Document type: Application note
- Page count: 8
- Read time: 9 minutes