Poster Presentation: High-Throughput Quantification of Antibody-Dependent Phagocytosis using Live-Cell Analysis
Presented at the 2022 American Association of Immunologists (AAI) Annual Meeting
Overview
The uptake and clearance of tumor cells can be promoted with monoclonal antibodies (mAbs) via antibody-dependent phagocytosis (ADCP) or through the blockade of “don’t-eat-me” signals, such as CD47. These mechanisms hold immunotherapeutic promise and are studied extensively in drug development.
High-throughput assays were conducted using pHrodo®, a pH-sensitive fluorophore, that enables live-cell imaging and kinetic quantification of phagocytosis. pHrodo® labeled target cells were incubated with mAbs and added to effector cells in 96-well plates. Images were acquired using the Incucyte® Live-Cell Analysis System and fluorescence automatically quantified with integrated software.
The data presented in this poster exemplifies that live-cell analysis is a powerful approach that enables functional quantification of ADCP and is amenable to antibody screening for therapeutic candidates.
Speaker Bio
Jasmine Trigg | Scientist, Sartorius
Jasmine is currently a Scientist at Sartorius as part of the Cell Imaging and Applications group within the Bio Analytics team, where she is involved in the research and development of novel applications for the Incucyte® Live-Cell Analysis Systems.
Over the past couple of years, she has worked across multiple research areas developing cellular assays to extend the suite of live-cell analysis applications. Jasmine has a background in neuroscience and genetic manipulation and her earlier work focused on using a combined structural and molecular biology approach to assess disease-associated proteins implicated in Alzheimer’s disease.
