On-Demand Webinar: Live-Cell Imaging Strategies to Quantify Phagocytosis
Phagocytosis is a dynamic and complex set of processes for the internalization, destruction and removal of pathogens, dying and foreign cells from the body. It has relevance in many therapeutic areas, such as oncology, immuno-oncology, and neuro-inflammation. Phagocytosis is a specific form of cellular endocytosis, performed by immune cells, such as macrophages, neutrophils, immature dendritic cells and microglia.
One key function of phagocytosis is the clearance of apoptotic cells, termed efferocytosis, which is crucial in resolving inflammatory events, by the removal of neutrophils that remain following inflammation. Efferocytosis also prevents the development of chronic inflammation in disease and can remove apoptotic tumor cells following anti-cancer treatment.
Additionally, viable cells can be targeted with pro-phagocytic agents that promote engulfment and clearance through engaging host phagocytes. These mechanisms often involve the addition of an antibody to drive clearance, for example, antibody dependent cellular phagocytosis (ADCP) or the blockade of “don’t-eat-me” signals such as CD47.
Live-cell analysis methods have been developed using pH-sensitive florescent dyes, such as pHrodo®, that enable quantification of target cell efferocytosis, which is detected through an increase in fluorescence.
Key webinar discussion topics include:
- Quantification of phagocytosis using Incucyte® Live-Cell Analysis Systems.
- Guidance on experimental set-up and optimization, and how live-cell analysis can be built into development workflows.
- An overview of validated assays combining pHrodo® reagents with Incucyte® Live-Cell Analysis Systems, for image-based fluorescent readouts of phagocytosis.
- Example data to support the use of live-cell analysis within the fields of immuno-oncology, inflammation & infection and neuroscience.
About the speaker
Jasmine Trigg
Associate Scientist
Sartorius
Jasmine is currently an Associate Scientist at Sartorius as part of the cell imaging and applications group within the Bio Analytics team.
Over the past couple of years, she has worked across multiple research areas developing cellular assays to extend the suite of live-cell analysis applications.
Jasmine has a background in Neuroscience and Genetic Manipulation and her earlier work focused on using a combined structural and molecular biology approach to assess disease-associated proteins implicated in Alzheimer’s disease.
