Cell-based Phenotypic Screening With Incucyte® Live-Cell Analysis Systems
Authors: Gillian Lovell, Jasmine Trigg, John Rauch, Nicola Bevan | Last updated: August 2023
Overview
Phenotypic screening – the measurement of a defined output in cells exposed to a large number of chemical compounds – is a vital tool in drug discovery, enabling scientists to rapidly identify potential drugs early in the discovery pipeline. Live-cell imaging provides vastly more biological information than a single endpoint readout since images reveal insight into growth and morphology changes of cells which are maintained within a physiologically relevant environment.
Here we describe the application of Incucyte® Live-Cell Analysis Systems to derive insights into a library of 880 FDA-approved drugs by integrating data from label-free image analysis with quantification of fluorescent reporters.
- Document type: Application Note
- Page count: 10
- Read time: 10 minutes
Key Takeaways
- Explore how kinetic information gained by repeated image acquisition furthers insight into the dynamic changes which are often overlooked using end-point analysis alone
- Discover how combining multiple readouts elucidates compound mechanisms and aids in the removal of misleading results
- Learn more about how live-cell imaging demonstrates morphological changes yielding highly robust and insightful data
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Figure 1. Phenotypic screening using Incucyte® Live-Cell Analysis Systems provides insight into compound mechanisms of action
