Application Note: A Combined Live-Cell Analysis and HTS Cytometry Approach
Authors: Natasha Lewis, Jasmine Trigg, Daryl Cole, Nicola Bevan
Last updated: September 2024
Overview
Mesenchymal stem cells (MSCs) play a crucial role in regenerative medicine and cell therapy due to their ability to differentiate into multiple cell types and their immunomodulatory properties. Ensuring the quality of MSCs is essential for their effectiveness in therapeutic applications, especially in high-risk clinical settings.
Evaluating MSC quality is challenging due to factors like donor variability, culture conditions, and passage numbers. Traditional endpoint assays are insufficient for providing dynamic insights, highlighting the need for advanced technologies such as live-cell analysis and high-throughput screening (HTS) cytometry. These technologies offer real-time, high-throughput, and multiparametric analysis capabilities, effectively addressing these challenges.
- Document type: Application Note
- Page count: 8
- Read time: 10 minutes
Learn more about the importance of utilizing multiparametric techniques for a holistic view of MSC quality:
- How these techniques are used to demonstrate that MSC growth dynamics slow with age
- Why leveraging the strength of live-cell analysis researchers can benefit from comprehensive and high–resolution data for MSC biology
- Inform the optimization of culture conditions, passage strategies, and expansion protocols, ultimately leading to improved outcomes in MSC-based clinical applications
