Evaluating Organoid Culture Using Live-Cell Analysis and Sartorius RUO Cytokines

Authors: Natasha Lewis | Last updated: 27 September, 2023

Overview

Organoids have huge potential in the fields of research and medicine as they can accurately replicate both architecture and function of their full-scale counterparts. However, their full potential is limited by a lack of standardization, poor reproducibility, and challenging quantification of phenotypic data. Live-cell imaging allows the acquisition of images throughout the culture period in a non-perturbing manner, allowing preservation of precious material and comprehensive multiparametric analysis of growth dynamics.

In this webinar we demonstrate the use of the Incucyte® Organoid Analysis Software Module on a range of organoid types. We will also show how Sartorius Research Use Only (RUO) Growth Factors and Cytokines can be used to promote and sustain organoid growth and investigate the effects of individual growth factors on growth dynamics.


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Key Takeaways

Learn more about how:

  • The Incucyte® Live-Cell Analysis System enables non-perturbing observation of organoid growth dynamics during expansion and in assay formats
  • Standardized protocols alongside validated software increase reproducibility and preserve precious material
  • Sartorius RUO Growth Factors and Cytokines enable precise control of culture conditions and dissection of individual growth factor effects
  • Sartorius RUO Growth Factors and Cytokines can be used to maintain organoid growth and health in culture

Figure 1. Live-Cell Analysis Systems enabling non-perturbing observation of organoid growth dynamics during expansion and in assay formats

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