Immunotherapy Insights Through 3D Live-Cell Imaging

Confocal Live-Cell Imaging of 3D Immune Cell Killing | Application Note

Authors: Jasmine Trigg, Kirsty McBain, Gillian Lovell, Kalpana Barnes, John Rauch, and Nicola Bevan, Sartorius | Last updated: September 2026

 

Resource overview

Learn how the Incucyte® CX3 Live-Cell Analysis System enables real-time visualization and quantification of immune cell killing in 3D tumor multi-spheroid models using confocal live-cell imaging. Explore applications for CAR-T, NK cell, ADCC, and immuno-oncology research.


Key takeaways:

  1. Visualize immune cell infiltration and tumor cell killing in 3D spheroid models.
  2. Quantify immune-tumor interactions and apoptosis in real time.
  3. Evaluate CAR-T, NK cell, ADCC, and ADC responses with multiplexed imaging.
  4. Generate physiologically relevant data to support immuno-oncology research.

     

Resource details:

  • Document type: Application note
  • Page count: 13
  • Read time: 10 minutes

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