Resource overview
What if you could visualize the complete mechanism of action of an ADC in a single experiment? This application note shows how 3D spheroid and immune cell co-culture models, combined with multiplexed confocal live-cell imaging, provide a more physiologically relevant view of ADC activity. Explore how the Incucyte® CX3 uncovers key functional differences in internalization, tumor cell killing, ADCC, and bystander effects that may be missed by traditional in vitro approaches.
Key takeaways:
- Learn how to measure ADC internalization, cytotoxicity, ADCC, and bystander killing in a single 3D spheroid workflow
- Discover how multiplexed confocal live-cell imaging generates real-time mechanistic insights
- Explore how advanced 3D co-culture assays can support ADC characterization and candidate selection
Resource details:
- Document type: Application note
- Page count: 12
- Read time: 12 minutes
