Multi-Parametric Quantification of Monocytes using Live-Cell Analysis Poster
Authors: Jasmine Trigg | Last updated: March 2023
Overview
Monocytes play critical roles in innate immunity by migrating to inflamed tissue, where they clear micro-organisms and apoptotic cells, repair injured tissues, and recruit other immune cells. These highly plastic cells can change their functional phenotypes in response to a variety of cellular signals, including the Akt signaling pathway. Here we provide robust in vitro assays for the kinetic evaluation of monocytes and exemplify how these further our understanding of their biological roles.
The Incucyte® Live-Cell Analysis System was used to acquire phase and fluorescence images of monocytes, which were automatically analyzed using integrated software. Cytokine analysis was performed using the iQue® Advanced Flow Cytometry platform and a custom Qbeads® PlexScreen Kit.
These data exemplify that live-cell analysis, alongside advanced analytical methods, is a powerful approach enabling multi-parametric quantification of immune cells.
- Document type: Poster
- Page count: 1
- Read time: 5 minutes
Key Takeaways
- The Incucyte® Live-Cell Analysis system enables long-term imaging of monocytes and quantification using integrated software.
- Advanced analytical approaches, including morphological and AI-based classification, facilitate the robust segmentation and quantification of monocytes including morphological changes and functional readouts.
- The iQue® Advanced Flow Cytometry Platform and custom kits enables cytokine analysis of monocytic cultures.
- This powerful, multi-parametric approach benefits multiple research areas include immunology and immuno-oncology.
This Resource is Designed for:
- Immunologists
- Oncologists
- Cell biologists
- Microscopists
Applications Supported:
- Live-cell analysis
- Flow cytometry
- Label-free image analysis
- Chemotaxis
- ADCP
- Akt activation
