Minimizing Syringe Filter Usage in Harvesting Monoclonal Antibodies from CHO Cell Culture Supernatants
Authors: Michael Grauf, Klaus Schöne | Last updated: 27.04.2018
Overview
The clarification of cell culture supernatants with volumes of < 25 mL for harvesting monoclonal antibodies by using syringe filters is often a laborious and, sometimes, an exhausting step. Therefore, the selection of a suitable filter type is essential.
In this study, the performance of the Sartorius Minisart® High Flow filter for clarification of CHO cell culture supernatants was compared with a syringe filter type from another supplier, each with a similar effective filtration area. To obtain robust results, the study examined ten combinations of cultivation methods and monoclonal antibody products, such as IgG1, IgG2, fc fusion proteins and bispecific antibodies, for turbidity, mAb recovery, relative yield, and throughput.
- Document type: Application Note
- Page count: 4
- Read time: 11 minutes
Key Takeaways
- Sartorius Minisart® High Flow larger filtration area results in 94% more filtrate volume per unit, reducing filter usage.
- Choosing the right syringe filter for CHO cell culture clarification can improve sample throughput.
This Resource is Designed for:
- Lab Researchers
Applications Supported:
- CHO Cell Culture
- Clarification
- Supernatant
- Syringe Filter
- Turbidity
- Product Recovery
- Yield
- Throughput
- Filter Usage
