Flow Cytometry Reagents for Phenotype and Function

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Immunophenotyping and Function using iQue® Immune Cell-Based Kits

For Measuring Multiple Cellular End Points

iQue® Immune Cell-Based Kits enable the analysis of multiple immunological cell phenotypes and functional traits such as viability, proliferation, and cytokine release. Most of these kits are optimized for low-wash protocols.

Each of these are available in different sizes to meet your screening needs. The Forecyt® software has automated templates for most of these kits making it easy to acquire and analyze data. All have been optimized for use on the iQue® Screener platforms.

Available iQue® Phenotype and Function Kits include:

iQue® Human T Cell Activation Kit:

Single wash assay provides a multiplexed approach to measure T-cell activation, proliferation and quantify cytokine release in a single well.

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iQue® Human T Cell Companion Kit:

Simultaneously measure additional cytokines besides those provided in T Cell Immunology Kit.

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iQue® Human T Cell Memory Kit:

Analyze T cell memory phenotype and function at different stages with the new T Cell Memory Cell and Cytokine Profiling Kit.

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iQue® Human General Immune Cell Killing Kit:

Evaluate cellular phenotypic and functional markers and bead-based, secreted cytokine measurements in the same assay.

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iQue® Human T Cell Killing Kit:

Evaluate T cell immunophenotypes and function, cell health, cell count, secreted pro-inflammatory cytokines in a single assay.

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iQue® Mouse T Cell Kit:

Evaluate T cell activation, memory, exhaustion and cytokine secretion in the same well.

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iQue® Human T Cell Exhaustion Kit:

Multiplex T cell exhaustion, phenotyping T helper and T cytotoxic cells, and bead-based measurement of secreted cytokines, all in the same assay.

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iQue® Human NK Cell Killing Kit:

Measure activation markers, cell membrane integrity, cell count, secreted pro-inflammatory cytokines, and pro-apoptotic protease from a single assay.

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iQue® Human T Cell Phenotyping Kit:

Evaluate phenotype and function of multiple of T subsets in the same well.

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iQue® Human T Cell Activation Kit

iQue® Human T Cell Activation Kit

The iQue® Human T Cell Activation Cell and Cytokine Profiling Kit assesses the activation status of T cells while providing information about their health and their role in cytokine secretion. This one wash assay requires minimal hands on time and measures both cells and beads to provide the following biological insights:

  • Live immune cells are distinguished from dead cells by staining with a fluorescent membrane integrity dye.
  • Cells are immunophenotyped by staining with a fluorescent antibody panel to separate CD3+ T cells, CD3- non-T cells, CD4+ T helper cells, CD8+ T cytotoxic cells.
  • The T cell phenotypes are profiled for the expression of 3 activation markers, CD69 (early), CD25 (late), and HLA-DR (even later, with strong stimulation).
  • Optimized workflow enables the measurement of effector cytokines secreted by activated T cells, Th1 cytokine IFNγ/ multifunctional cytokine TNFα in the same assay well.

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Key Benefits

  • Speed: Sample to actionable data by allowing multiple assays to be run in one assay reducing the hands on time, numbers of overall tests and conserving precious sample.
  • Results: 15 endpoints from a single well assay measuring T cell phenotypes, early and late cell activation markers in each phenotype, cell viability, cell count, proliferation and secreted effector cytokines.
  • Biological insight: Generate cell health and functional data from same cell by multiplexing cell and cytokine detection in one well.
  • Simplicity: Single platform assay and data acquisition eliminates the time of acquiring and correlating data from multiple sources.
  • Automation friendly: Compatible for automated high-throughput screening with excellent day-to-day reproducibility.


Workflow

Human T Cell activation kit workflow image


iQue® Human T Cell Activation Kit Ordering Information

Platform

Compatible with iQue® 3/iQue® Screener Plus - VBR Configuration

Available Sizes

Catalog Numbers

1 x 96 well

90560

5 x 96 wells

90561

1 x 384 wells

90562

5 x 384 wells

90563

iQue® Human T Cell Companion Kit

iQue® Human T Cell Companion Kits

The iQue® Human T Cell Companion Kits can be used to measure additional cytokines besides those already included in the iQue® Human T Cell Activation Cell and Cytokine Profiling Kit or other T Cell Immunology Kits. Below is the list of available cytokines for T Cell Companion Kits.

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iQue® Human T Cell Companion Kit Ordering Information

DescriptionCatalog no.
iQue® Human IL-2 T Cell Companion Kit

97028

iQue® Human IL-6 T Cell Companion Kit

97029

iQue® Human IL-10 T Cell Companion Kit

97030

iQue® Human IL-13 T Cell Companion Kit

97031

iQue® Human IL-17A T Cell Companion Kit

97032

iQue® Human GM-CSF T Cell Companion Kit

97033

iQue® Human TNF T Cell Companion Kit

97034

iQue® Human T Cell Memory Kit

iQue® Human T Cell Memory Kit

The iQue® Human T Cell Memory Kit measures T cell memory phenotype and function at different stages while providing information about their health and their role in cytokine secretion. This one wash assay requires minimal hands on time and measures both cells and beads to provide the following biological insights:

  • Live immune cells are distinguished from dead cells by staining with a fluorescent membrane integrity dye.
  • Cells are immunophenotyped by staining with a fluorescent antibody panel to separate CD3+ T cells, CD3- non-T cells, CD4+ T helper cells, CD8+ T cytotoxic cells.
  • The panel also includes 5 different T cell surface markers for T naive/memory/effector cell phenotyping: CD45RA, CD45RO, CD27 (long term survival), CD62L, and CD95.
  • Optimized workflow enables the measurement of effector cytokines secreted by activated T cells, including pro-inflammatory cytokine IFNγ and anti-inflammatory cytokine IL-10 in the same assay well.

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Key Benefits

  • Speed: Sample to actionable data by allowing multiple assays to be run in one assay reducing the hands on time, numbers of overall tests and conserving precious sample.
  • Results: 11 endpoints from a single well assay measuring T cell memory phenotype and function at different stages, cell viability, cell count, and secreted pro/anti-inflammatory cytokines.
  • Biological insight: Generate cell health and functional data from same cell by multiplexing cell and cytokine detection in one well.
  • Simplicity: Single platform assay and data acquisition eliminates the time of acquiring and correlating data from multiple sources.


Workflow

human t-cell workflow


iQue® Human T Cell Memory Kit Ordering Information

Platform

Compatible with iQue® 3/iQue® Screener Plus - VBR Configuration

Available Sizes

Catalog Numbers

1 x 96 well

97035

5 x 96 wells

97036

1 x 384 wells

97037

5 x 384 wells

97038

iQue® Human General Immune Cell Killing Kit

iQue® Human General Immune Cell Killing Kit

The iQue® Human General Immune Cell Killing Kit was designed for ease of use in multiplexing cellular phenotypic and functional markers and bead-based, secreted cytokine measurements in the same assay. This no wash assay requires minimal hands on time and measures both cells and beads to provide the following biological insights:

  • Live immune cells are distinguished from dead cells by staining with fluorescent membrane integrity dye and mitochondrial membrane potential dye.
  • Target cells are distinguished from effector cells by staining with a fluorescent encoder dye.
  • Simultaneous analysis of multiple mechanisms of immune cell mediated cell killing in a single, high content assay including mitochondrial membrane depolarization, loss of cell membrane integrity, cell count, and pro-apoptotic protease Granzyme B.

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Key Benefits

  • Speed: Expedient sample to actionable data allows multiple assays to be run in one experiment thereby reducing the hands on time, required numbers of overall tests and conserving precious sample.
  • Results: Five endpoints from a single well assay measure target and effector cells, mitochondrial membrane depolarization, cell membrane integrity, cell count, and secreted pro-apoptotic protease Granzyme B.
  • Biological insights: Investigation of multiple mechanisms of immune cell mediated cell killing in one well.
  • Ease of use: Single platform assay and data analysis with iQue Forecyt® eliminates the time required for acquiring and correlating data from multiple sources.


Workflow

human general immune cell mediated killing kit workflow


iQue® Human General Immune Cell Killing Kit Ordering Information

Platform

Compatible with iQue3/iQue Screener PLUS VBR and BR configuration

Available Sizes

Catalog Numbers

1 x 96 well

97076

5 x 96 wells

97077

1 x 384 wells

97078

5 x 384 wells

97079

iQue® Human T Cell Killing Kit

iQue® Human T Cell Killing Kit

The iQue® Human T Cell Killing Kit (CD3, CD4, and CD8) is designed for reliable identification of human T cell subsets. This assay is optimized to run on the iQue® 3 (VBR and VYB configurations) platform combining high throughput sampling, flow cytometry detection, and multiplexing capabilities.

  • T cells are immunophenotyped by staining with a fluorescent antibody panel to separate CD3+, CD4+, and CD8+ cells.
  • Compatible with Qbead Panels enabling cytokine detection and cell phenotyping at the same time.
  • Optimized workflow provides flexibility by enabling additional cytokines to be added for further characterization of subpopulations.

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Key Benefits

  • High-throughput: Designed for high-throughput immunophenotyping of T cells in 96- and 384-well plate format using 10µL (96-well format) and 5µL (384-well format) of cells.
  • Biological insights: Generate functional data and option to analyze cell health from same cell by multiplexing cell phenotypes and function in a single well.
  • Flexibility: Choose additional cytokines to be added for further characterization of subpopulations.


Workflow

human t cell mediated killing kit workflow


iQue® Human T Cell Killing Kit Ordering Information

Platform

Compatible with iQue® 3/iQue® Screener Plus - VBR Configuration

Available Sizes

Catalog Numbers

1 x 96 well

97060

5 x 96 wells

97061

1 x 384 wells

97062

5 x 384 wells

97063

iQue® Mouse T Cell Kit

iQue® Mouse T Cell Kit

The iQue® Mouse T Cell Kit measures T cell activation, memory, exhaustion while providing information about their health and their role in cytokine secretion. This one wash assay requires minimal hands on time and measures both cells and beads to provide the following biological insights:

  • Live immune cells are distinguished from dead cells by staining with a fluorescent membrane integrity dye.
  • Cells are immunophenotyped by staining with a fluorescent antibody panel to separate CD3+ T cells, CD3- non-T cells, CD4+ T helper cells, CD8+ T cytotoxic cells.
  • The panel also includes 4 different T cell surface markers for T cell activation, memory and exhaustion in mouse.
  • Optimized workflow enables the measurement of effector cytokines secreted by T cells, including pro-inflammatory cytokine IFNγ and anti-inflammatory cytokine IL-2 in the same assay well.

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Key Benefits

  • Speed: Sample to actionable data by allowing multiple parameters to be assessed in one assay reducing the hands on time, numbers of overall tests and conserving precious sample.
  • Results: 11 endpoints from a single well assay measuring T cell activation, memory, exhaustion, cell viability, cell proliferation, and secreted pro/anti-inflammatory cytokines in mouse.
  • Biological insight: Generate cell health and functional data from same cell by multiplexing cell and cytokine detection in one well.
  • Simplicity: Single platform assay and data acquisition eliminates the time of acquiring and correlating data from multiple sources.


Workflow

mouse t cell and cytokine profiling kit workflow


iQue® Mouse T Cell Kit Ordering Information

Platform

Compatible with iQue3/iQue Screener PLUS VBR configuration

Available Sizes

Catalog Numbers

1 x 96 well

97042

5 x 96 wells

97043

1 x 384 wells

97044

5 x 384 wells

97045

iQue® Human T Cell Exhaustion Kit

iQue® Human T Cell Exhaustion Kit

The iQue® Human T Cell Exhaustion Kit is designed for ease of use in multiplexing markers of T cell exhaustion, phenotyping T helper and T cytotoxic cells, and bead-based measurement of secreted cytokines, all in the same assay. This optimized assay offers these unique advantages:

  • Live immune cells are distinguished from dead or membrane compromised cells by staining with a fluorescent membrane integrity dye.
  • Cells are immunophenotyped by staining with a fluorescent antibody panel to distinguish CD3+ T cells, CD3- non-T cells, CD3+CD4+ helper T cells, and CD3+CD8+ cytotoxic T cells.
  • The panel also includes three markers of T cell exhaustion: PD-1, Tim-3, and Lag-3.
  • Optimized workflow enables the measurement of cytokines secreted by activated T cells (IFNγ and TNF) in the same assay well.
  • The capacity of the sample cells to proliferate can also be determined with an optional proliferation dye if desired (included with the kit).

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Key Benefits

  • Speed: Sample to actionable data by allowing multiple assays to be run in one assay thereby reducing the hands on time, numbers of overall tests and conserving precious sample.
  • Results: 11 endpoints from a single-well assay measuring T cell exhaustion phenotypes and function, as well as cytokine secretion in response to stimulation, cell viability, and an optional proliferation dye allows screening for molecules that reverse T cell exhaustion.
  • Biological insight: Generate functional data and analyze cell health from the same well by multiplexing cell and cytokine detection in one assay.
  • Simplicity: Single platform assay and data acquisition and analysis eliminates the time of acquiring and correlating data from multiple sources.


Workflow

human t cell exhaustion cell and cytokine profiling kit workflow


iQue® Human T Cell Exhaustion Kit Ordering Information

Platform

Compatible with iQue3/iQue Screener PLUS - VBR configuration

Available Sizes

Catalog Numbers

1 x 96 well

97069

5 x 96 wells

97070

1 x 384 wells

97071

5 x 384 wells

97072

iQue® Human NK Cell Killing Kit

iQue® Human NK Cell Killing Kit

The iQue® Human NK Cell Killing Kit was designed for ease of use in multiplexing cellular phenotypic and functional markers and bead-based, secreted cytokine measurements in the same assay. This one wash assay requires minimal hands on time and measures both cells and beads to provide the following biological insights:

  • Live immune cells are distinguished from dead cells by staining with fluorescent membrane integrity dye.
  • Target cells are distinguished from effector cells by staining with a fluorescent encoder dye.
  • NK cells are immunophenotyped by staining with a fluorescent antibody panel to separate CD3- CD56+ NK cells. The expression of the IgG Fc receptor III (CD16) on the NK cells can then be assessed.
  • The panel also includes 2 different NK cell functional activation markers, CD69 and CD25.
  • Optimized workflow enables the measurement of pro-inflammatory effector secreted cytokine IFNγ and pro-apoptotic serine protease Granzyme B in the same assay well.

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Key Benefits

  • Speed: Sample to actionable data allows multiple assays to be run in one experiment thereby reducing the hands on time, required numbers of overall tests and conserving precious sample.
  • Results: Nine endpoints from a single well assay measuring activation markers (CD69 and CD25), cell membrane integrity, cell count, secreted pro-inflammatory cytokine IFNγ, and pro-apoptotic protease Granzyme B.
  • Biological insights: Generate functional data and analyze cell health from same cell by multiplexing cell phenotypes and function in a single well.
  • Ease of use: Single platform assay and data analysis with ForeCyt® eliminates the time required for acquiring and correlating data from multiple sources.
  • Flexibility: Choose additional cytokine measurements from a validated list of iQue® Human NK Cell Companion Kits.


Workflow

human nk cell mediated killing kit workflow


iQue® Human NK Cell Killing Kit Ordering Information

Platform

Compatible with iQue3/iQue Screener PLUS VBR configuration

Available Sizes

Catalog Numbers

1 x 96 well

97082

5 x 96 wells

97083

1 x 384 wells

97084

5 x 384 wells

97085

iQue®️ Human T Cell Phenotyping Kit

iQue® Human T Cell Phenotyping Kit (CD3, CD4 and CD8)

The iQue® Human T Cell Phenotyping Kit (CD3, CD4 and CD8) is designed for reliable identification of human T cell subsets. This assay is optimized to run on the iQue® 3 (VBR and VYB configurations) platform combining high throughput sampling, flow cytometry detection and multiplexing capabilities.

  • T cells are immunophenotyped by staining with a fluorescent antibody panel to separate CD3+, CD4+ and CD8+ cells.
  • Compatible with Qbead Panels enabling cytokine detection and cell phenotyping at the same time.
  • Optimized workflow provides the flexibility by enabling additional markers and cytokines to be added for further characterization of subpopulations.

Request a Quote


Key Benefits

  • High-throughput: Designed for high-throughput immunophenotyping of T cells in 96- and 384-well plate format using 10µL (96-well format) and 5µL (384-well format) of cells.
  • Biological insights: Generate functional data and option to analyze cell health from same cell by multiplexing cell phenotypes and function in a single well.
  • Flexibility: Choose additional markers and cytokines to be added for further characterization of subpopulations.


Workflow

iQue t cell phenotyping kit workflow


Data Analysis

iQue t cell phenotyping kit analysis

Phenotyping analysis of human PBMCs cultured for one day. A. Set gate for live cells in all events to exclude debris/dead cells. B. Remove doublets from live cells to obtain single cells. C. CD3+ cells of Singlets. D. CD4+ and CD8+ subsets of CD3+ cells.


iQue® Human T Cell Phenotyping Kit (CD3, CD4 and CD8) Ordering Information

Platform

Compatible with iQue3/iQue Screener PLUS VBR/VYB configurations

Available Sizes

Catalog Numbers

1 x 96 well

97099

5 x 96 wells

97100

1 x 384 wells

97101

5 x 384 wells

97102

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