Application Note: Secreted Protein Release in Inflammatory and Neuroinflammatory Models
Authors: Jasmine Trigg, Kirsty McBain, Daryl Cole and Nicola Bevan|
Last updated: February 2025
Overview
Studies have shown that inflammation not only plays a crucial role in advancing neurodegeneration, but can also act as initiator, with inflammatory signaling exhibiting beneficial and detrimental roles, depending on the stage of a disease.
The understanding of immune cell activation and complex cellular signaling cascades, involved in neuroinflammation and neurodegeneration, is facilitated by the quantification of biologically relevant, secreted chemokines and cytokines. This provides crucial information during basic research and drug development.
iQue Qbeads® enable the capture of specific proteins on distinct bead types, and facilitate the multiplexed quantification of a broad range of cytokines, adhesion molecules, enzymes, and growth receptors. These ready-to-run, pre-built or custom kits, have been developed for use on the iQue® High-Throughput Screening (HTS) Cytometry Platform, provide an integrated solution with unique benefits of simple no-wash protocols, a high degree of assay miniaturization, rapid sampling speeds, and cost efficiencies.
This application note demonstrates how a combination of iQue Qbead® kits and HTS by Cytometry can be utilized to simultaneously quantify multiple secreted proteins in inflammatory and neuroinflammatory models.
Also described, is how this flexible approach can be used to investigate iPSC-derived neuronal models
- Document type: Application Note
- Page count: 12
- Read time: 12 minutes
Key Takeaways
This application note contains detailed sections on:
- Secreted Protein Release in Inflammatory Models of Akt Activity
- Utilizing HTS Cytometry to Assess Phenotypic Differences Following Macrophage Polarization
- Neuroinflammatory Protein Release in Healthy and Diseased iPSC-derived Models
