Incucyte® Nuclight Rapid Red Dye Compatibility with CellCelector
Authors: Daryl Cole, Scientist, Sartorius | Last updated: August 2025
Overview
This technical note explores the compatibility of Incucyte® Nuclight Rapid Red Dye with the CellCelector Flex, emphasizing their combined use in cell proliferation and cytotoxicity assays. The CellCelector Flex isolates single cells, while the Incucyte Live-Cell Analysis System monitors cell growth and morphology.
The Nuclight Rapid Red Dye labels cell nuclei effectively without affecting growth, enabling real-time proliferation quantification. Experiments successfully identified HER2 positive breast cancer AU565 cells using fluorescence imaging. This integration enhances drug discovery, cancer research, and cell culture optimization by providing comprehensive phenotypic characterization, confirming the dye's efficacy for precise cell identification in developmental research and cytotoxicity monitoring.
Document Type: Technical Note
Page Count: 4
Read Time: 8 Minutes
Key Takeaways
- Compatibility and integration
- Real-time monitoring
- Successful cell identification
- Enhanced research applications
