Technical Note: CellCelector Nanowell Arrays
Last updated: December 2024
Overview
The CellCelector Flex is a flexible and powerful system providing automated solutions to numerous technically challenging experimental procedures that are conventionally undertaken manually. Single cell screening and isolation, and single cell cloning workflows can be performed on the system using a wide range of CellCelector nanowell arrays for both clinical research and bioprocessing applications.
By integrating these nanowell solutions with the CellCelector, you gain access to high-throughput, cost-effective alternatives to traditional methods like limiting dilution and FACS. Sartorius offers nanowell arrays in multiple formats, from 2 well chamber slides to 6 and 24 well plates, featuring between 100,000 to several million nanowells per plate or slide, with volumes ranging from several nanoliters to a few picoliters. This technical note explores the applications of these nanowell arrays and highlights specific protocols that incorporate this technology to streamline workflows and optimize resource use.
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- Document type: Technical Note
- Page count: 6
- Read time: 6 minutes
Key Takeaways
- Single Cell Cloning
- Single Cell Screening
- Rare Single Cell Isolation
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CellCelector Nanowell Plates
Micrographs illustrating S200 nanowell plate structure with up to 4,000 individual nanowells per well of a 24-well plate format array. iPSCs were seeded as single cells and incubated for 4 days until iPSC spheroids formed. The CellCelector identified nanowells containing one cell at seeding to ensure monoclonality of iPSC spheroids.
