Head-To-Head Data: PFVs, LNPs, and Electroporation for Nucleic Acid Delivery in Cell Engineering
On-Demand Webinar
Can pre-formed vesicles (PFVs) deliver better outcomes than electroporation and lipid nanoparticles (LNPs) for nucleic acid delivery in cell engineering? This session presents head-to-head comparative data across delivery platforms, covering transfection efficiency, cell viability, yield, and scalability across T cell, NK cell, and other clinically relevant cell types.
Gain a practical framework for selecting the most appropriate delivery platform based on payload type, cell type, and manufacturing context, with data drawn from mRNA, CRISPR, and DNA delivery applications in both centralized and decentralized manufacturing settings.
What You Will Learn:
- Review comparative data demonstrating greater than 80% knockout efficiency with PFVs versus electroporation, a more than 5-fold increase in edited T cell yield by day 10, and over 85% CAR+ T cells achieved with PFVs compared to approximately 40% with LNPs
- Understand how PFVs support scalable, closed manufacturing workflows, including more than 80% TCR knockout efficiency in stirred-tank bioreactor systems and more than 30% stable gene insertion in primary human T cells using a fully non-viral approach
- Evaluate practical criteria for selecting between PFVs, LNPs, and electroporation based on cell type, payload, and workflow requirements
- Apply instrument-free, incubation-based PFV loading to reduce formulation complexity, shorten optimization cycles, and accelerate progression from research to clinic
