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Viral Vector Upstream Processing Course (VV-USP)

Cycle 5

This course will provide you with the necessary scientific background on viral vectors, giving an overview of the essential steps in the upstream processing of vectors, including analytical tools, process development considerations and manufacturing strategies, focusing primarily on AAV, LV and AdV. It will also offer insight into the market and trends as well as industry needs and challenges.

It includes:

  • Mix of self-paced and instructional units with 4 - 5 hours of learning per week (35 – 40 hours in total) over 2.5 months
  • Interactive collaborative platform to facilitate knowledge exchange
  • Limited synchronous activities allow participation from across the globe
  • 98% recommendation rate from previous learners


Who Should Attend

Lab managers, team leads, researchers, engineers and scientists with expertise in bioprocessing and basic knowledge of gene therapy. Fluent English is essential for optimal learning.


What You Will Learn

Upon completion of this course, you will be able to:

  • Understand various forms of viral vectors and their application
  • Differentiate between various cell lines used and understand their testing and regulatory requirements
  • Explain upstream process development considerations (parameters, cell line, media, scale-up) and tools used
  • Discuss production strategy, modes, workflows along with process controls and analytics

How You Will Learn

This online course consists of both self-paced and collaborative learning units, allowing you to fit in learning around your work schedule. Each chapter includes live discussions, assignments, access to experts and Q&A sessions, using an interactive and collaborative platform to facilitate knowledge exchange between participants. 

Focus Topics & Agenda

How is the Sartorius Academy’s Viral Vector Upstream Course structured?

Oct 31


Nov 07-Nov 18

Introduction to Viral Vectors

Nov 21-Dec 02

Cell Line Development and Cell Banking

Dec 05-Dec 16

Process Development and Manufacturing

Dec 19-Jan 06


Jan 09-Jan 20

Analytical Methods

Feb 03-Feb 04


Learning Modules

Duration: 2.0 weeks

  • Viral vector market, trends and opportunities
  • Description of the Adeno-associated virus, Lentivirus and Adenovirus
  • The basics of the viral vector manufacturing process

Duration: 2.0 weeks

  • Review of typical cell line used for viral vector production
  • Basics about transient production (transfection, infection), and stable production
  • Introduction to cell banking, virus banking, testing required and regulatory considerations

Duration: 2.0 weeks

  • Overview of AAV, LV and AdV upstream workflows
  • Review of key process development considerations: cell culture optimization, adherent vs suspension production, process optimization and scale-up
  • Review the different production strategies for viral vectors
  • Process controls and analytics during production

Duration: 2.0 weeks

  • Where and why analytics are used in viral vector processes
  • Introduction to the commonly used analytical solutions
  • Considerations and challenges to select the right analytical methods

How to Apply


Pricing and Special Rates

Registration fee: €2,500 + applicable taxes

Special rates may be considered for group bookings, public and academic institutes.

Contact for further information.

For terms & conditions, click here



Application closes 17 October 2022. Successful applicants will be informed by 24 October 2022. 20 seats are reserved for participants from the biopharmaceutical industry.


How to Apply

Please complete the registration form below. A maximum of 30 participants will be accepted to the course, which will be made up of co-learners from both Sartorius and industry.

Any questions prior to registration should be directed to

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