Advanced Strategies for Effective mRNA Transfection in T Cells
On-Demand
Overview
Optimize your workflows and enhance your therapeutic potential using mRNA transfection in human T cells. In this webinar, experts from Sartorius share cutting‑edge approaches that maximize transfection efficiency, promote cell expansion, and support potent anti‑tumor activity — all in a streamlined, non‑viral system.
Why Watch?
- Learn techniques to maintain T-cell vitality and support proliferation during and after mRNA delivery.
- Discover how live‑cell imaging and high‑throughput bioanalysis (e.g. Incucyte®, iQue®platforms) can guide optimization of transfection workflows
- Evaluate functional outcomes: cytotoxic potential of engineered T cells against target cancer cell lines such as A549
In This Webinar You Will Learn:
- Media & Activation Strategies – The influence of specialized Nutri‑T GMP media, IL‑2 vs IL‑21 cytokine usage, and TransAct activation approaches for robust recovery and expansion during transfection
- Transfection Optimization with jetMESSENGER® – Dosage ratios, reagent‑mRNA complex formation, timing, and techniques for minimizing cytotoxic impact while maximizing expression (e.g., eGFP mRNA model system)
- Kinetic Monitoring & Analytics – Using real‑time live‑cell imaging (Incucyte®) combined with high‑throughput cytometry (iQue®) to track viability, activation markers, and transfection efficiency across timepoints
Functional Validation – Demonstrated immune‑cell killing assays: transfected T cells efficiently target and eradicate red‑fluorescent A549 cancer cells in co‑culture assays
Watch now to transform your T‑cell engineering workflows: optimized for vitality, efficiency, and anti‑tumor potency.
Webinar Speaker
Hinnah Campwala, PhD
Principal Scientist,Sartorius
Hinnah Campwala is a Principal Scientist in the (Product Development) Reagent and Consumables Group at Sartorius. With a background in drug discovery and immune cell biology, she specializes in the development of innovative reagents and applications to support researchers working with Sartorius’s advanced bioanalytical tools.
Claire Guéguen, PhD
R&D Life Sciences Manager,Sartorius PolyPlus
Hinnah Campwala is a Principal Scientist in the (Product Development) Reagent and Consumables Group at Sartorius. With a background in drug discovery and immune cell biology, she specializes in the development of innovative reagents and applications to support researchers working with Sartorius’s advanced bioanalytical tools.