Application Note: Optimizing mRNA Transfection of Human T cells
Overview
Optimizing gene-delivery strategies for T-cells is essential to enhance their therapeutic potential. This study aimed to refine the transfection of human T-cells with mRNA using the jetMESSENGER® transfection reagent.
The specific objectives within this study were to identify culture conditions that maximize cell proliferation, transfection efficiency, and cytotoxic activity against cancer cells. To support T-cell recovery and expansion, the cells were cultured in specialized 4Cell® Nutri-T GMP media, enriched with high-quality cytokines from Sartorius. For kinetic visualization and quantitative assessments, which guided the optimization process, the Incucyte® Live-Cell Analysis System and the iQue® High-Throughput Screening (HTS) cytometer were utilized.
Document type: Application Note
Page count: 10
Read time: 14 minutes
Last updated: November 2024
Download the Application Note and Learn:
- Strategies for ensuring T-cell health and expansion during transfection
- Parameters to evaluate alongside cell health assessments
- Evaluating efficacy of tumor cell killing in transfected T cells
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