Troubleshooting HPLC Mobile Phase Issues
The HPLC mobile phase impacts retention and selectivity of analytes. Therefore, mistakes in mobile phase design and preparation will lead to considerable problems. This on-demand webinar will cover the areas of mobile phase preparation and design that are most likely to result in method errors and ensure that you have the tools to identify problems before they occur.
Topics include:
- Overview of mobile phase
- Flow rate
- Importance of pH
- Typical components
- Buffers (type, pH, molarity)
- Organic
- Ion pairing agents
- Mobile phase preparative errors (pH adjustment, mixing, filtration, degassing)
- Gradient elution (overview of effect of 5 main parameters, differences in pump gradient mixing, dwell volume)
Key Learning Objectives:
- How to be proactive to avoid common mistakes with HPLC
- Different requirements and preparation for mobile phases
- Tools that are needed to prevent HPLC problems
This webinar was used in the context of LCGC’s CHROMacademy. Don't miss out on this opportunity to enhance your HPLC knowledge.
Webinar Speaker
Colin Towers
Senior Technical Support Chemist
Element
After completing a degree in pharmacy, Colin Towers pursued a career in analytical chemistry. Following three years performing routine testing and three years instrument maintenance and calibration, he moved into a role as a method development and validation specialist in contract research. Gaining 11 years’ experience in developing methods for HPLC, LC-MS, GC, GC-MS and SPE in a high throughput commercial environment, this was supplemented by three years lecturing in forensic analytical toxicology and a further three years working as a community pharmacist. Towers has now been with Crawford Scientific for eight years as a technical specialist, consultant, and trainer.
