In Situ Hybridization: The Importance of Ultrapure Water for RNA Technologies
Authors: Frauke Nitzki , Elmar Herbig | Last updated: June 2024
Overview
In situ hybridization (ISH) for localization of DNA or RNA hybrids in cytological preparations was first described in 1969 by Gall and Pardue. This method enables mRNA transcripts to be detected in tissue sections. Unlike expression analyses based on polymerase chain reactions, the exact localization of the target transcripts can be identified within the tissue.
This article discusses the critical role of ultrapure water in ISH for RNA technologies, highlighting the Arium® Pro VF system's capabilities. It details the ISH process, the importance of preventing RNA probe degradation, and presents experimental results from tumor samples in genetically modified mice, which is ideal for researchers in human genetics and laboratory staff.
- Document type: Application Note
- Page count: 6
- Read time: 7 minutes
Key Takeaways
- Understand the importance of ultrapure water
- Explore a detailed overview of the ISH process
- Learn how Arium® Pro VF system produces ultrapure water by removing contaminants of different types from pretreated drinking water
- Discover results from ISH experiments on tumor samples from genetically modified mice
This Resource is Designed for:
- Lab Managers
- Scientists
- Biomedical Researchers
- Lab Technicians
- Analytical Lab
- Quality Control
- Molecular Biologists and Geneticists
- Environmental Scientists
Applications Supported:
- Life Science Research
- RNA Technologies
- Production of Ultrapure Water
- Cancer Research
