Live-Cell Monitoring: Optimizing Workflows for Advanced Cell Models
Authors: Sartorius | Last updated: June, 2025
Overview
As cell-based models become more advanced and essential in areas like drug discovery, immunology, neuroscience, and regenerative medicine, the need for continuous, dynamic monitoring has never been greater.
This white paper explores how real-time live-cell analysis enables scientists to optimize every phase of their assay development — from culture to manipulation to readout.
Download this resource to learn how to:
- Confidently QC complex cell cultures like organoids, spheroids, and iPSC-derived models
- Capture and quantify dynamic events such as differentiation, activation, and colony formation
- Improve reproducibility and reduce assay failure risk with continuous, non-invasive monitoring
- Leverage label-free and multiplexed imaging to detect subtle phenotypic changes
- Apply live-cell analysis across applications in immunology, oncology, and neurodegenerative disease research
Whether you're developing disease models, screening therapeutic candidates, or engineering cell lines, this white paper provides practical strategies to ensure the reliability, scalability, and translational value of your assays.
Key Takeaways
Live-cell imaging is vital for complex assay QC: Continuous, non-invasive monitoring detects subtle or unexpected changes in models like iPSCs, organoids, and spheroids—boosting reproducibility and reducing risk.
Real-time insights guide better decisions: Dynamic monitoring helps identify ideal timepoints for manipulations like differentiation, activation, or readouts.
Label-free, multiplexed methods offer deeper insights: Combining phase-contrast, fluorescent markers, and immunocytochemistry provides rich, multiparametric data on cell behavior.
Applicable across disciplines: From stem cell reprogramming to disease modeling and immunotherapy, live-cell monitoring supports workflows in oncology, immunology, and neuroscience.
Improves 3D and iPSC model consistency: As patient-derived models grow in complexity, live-cell QC ensures viability, uniformity, and accurate differentiation over time.
Enables smarter, more efficient assay development: Early insights reduce dependency on endpoint methods—saving time, resources, and cells.
Live-Cell Monitoring: Optimizing Workflows for Advanced Cell Models
