White Paper: Live-Cell Immunocytochemistry
Correlating surface marker expression with morphological and functional changes in living cells over time
Last updated: 8 July 2021
Overview
Immunocytochemistry (ICC) is a powerful laboratory imaging technique for visualizing the location of specific proteins in cells using targeted fluorescently-labeled antibodies (‘immunofluorescence’).
In this article, we introduce an integrated solution for ‘live-cell’ ICC, based on Incucyte® live-cell analysis and fluorescently-labelled antibody fragments. The methodology is amenable to any protein expressed on the cell surface to which there is a corresponding specific antibody.
In brief, the antibody is first tagged with an isotype-matched Fc-targeted fluorescently-labeled antigen binding fragment (Fab) via a simple mix, no wash protocol. This complex is then added directly to living cells and used to visualize the expression of proteins on cells.
Key Takeaways
Learn more about:
- a combined approach with analyses of other parameters such as cell health, neurite outgrowth and phagocytosis
- dynamic monitoring of surface proteins in living cells over several days
- monitoring cell-cell interactions
