Article Collection: Live-Cell Imaging and Analysis for Neuroscience
Last updated: 13 April 2023
Overview
Brains are complex organs, comprising numerous cell types that interact with each other in a myriad of ways. The most fundamental unit of interaction is at the level of the synapse, which has expanded beyond just the pre- and postsynaptic compartments of two connecting neurons to include modulation by associated astrocytes and surveillance, as well as potential pruning by microglia. The ability to study these interactions in a reductionist manner is key to understanding their role in physiology and pathology.
This article collection aims to help you gain a deeper understanding of how live-cell imaging can elucidate cellular mechanisms in neuronal and non-neuronal cell types, leading to development of more translatable models and facilitating development of potential therapeutic interventions.
Explore recent case studies exemplifying the power of live-cell imaging for neuroscience and the development of therapeutics.
- Document type: Article Collection
- Page count: 72
- Read time: 80 minutes
Key Takeaways
Learn more about:
Live-cell imaging of cultured neurons and glia
Automated measurements of cell morphology and neurite outgrowth
Utility of time lapse imaging for assessing cellular function
Quick Guide of Neurite Outgrowth Assay Workflow for Both Mono-culture and Co-culture Models
Figure 1. This simplified workflow enables label-free quantification of neurite dynamics in mono-culture or utilizes the Incucyte® Neurolight Orange Lentivirus for non-perturbing fluorescence analysis of neurons in co-culture. Using the Incucyte® Live-Cell Analysis System, visualize and quantify long-term changes in neurite outgrowth alongside optional readouts of cell viability using fluorescence cell death reporters.
