From Mammalian Cell Cultures to Pure Proteins: Sartoclear Dynamics Lab Significantly Reduces Cell Harvest Time
Application Note Overview
Sample preparation can be a time-consuming procedure. A particularly tedious step is centrifugation for separating mammalian cells. Therefore, we compared using the novel Sartoclear Dynamics® system with a standard centrifugation procedure as part of preparation of a transiently mammalian expressed Twin-Strep-tagged IgG.
Learn more about the study and how the Sartoclear Dynamics® system works by downloading our application note.
The study found that overall, the use of Sartoclear Dynamics® Lab:
Reduced the time for sample clarification significantly by up to 3.6-fold
Maintained the total protein yield and quality
Integrated easily into already existing lab processes
Decreased hands-on time significantly, thereby simplifying sample preparation
Figure above. Comparison of clarification methods by handling time. One liter of HEK293 cell culture each was clarified by the standard process (centrifugation) and by Sartoclear Dynamics® Lab with 40 g and 60 g filter aid, resp. Sartoclear Dynamics® Lab significantly reduces the time needed for clarification of HEK293 cell culture media