Poster: Characterize iPSC culture and differentiation with Advanced Flow Cytometry and Live-Cell Analysis
Authors: D. Cole, K. McBain, N. Bevan and T. Dale | Last updated: 23rd May 2023
Overview
iPSCs are a valuable resource in many areas of research and clinical development, however, highly specific conditions are required to maintain their pluripotency and viability. Long term iPSC cultures can develop genotypic and phenotypic heterogeneity, so it is vital that methods for detecting these changes are developed. Increasing use of stem cells in both clinical and research settings necessitates fast, reliable, and inexpensive solutions for iPSC culture and characterization. Here we demonstrate a streamlined workflow for the culture and characterization of iPSCs using Sartorius platforms for key attribute analysis.
- Document type: Poster
- Page count: 1
- Read time: 4 minutes
Key Takeaways
- A combined iQue and Incucyte workflow provides monitoring of key pluripotency attributes
- iPSCs can be monitored and characterized in both 2D and 3D over time
- Analyze iPSC cellular characteristics during differentiation towards a hepatic lineage
- Using Sartorius® RUO Growth Factors and Cytokines avoid daily stem cell media changes
