Comparative Analysis of anti-HER2 Antibody-Drug Conjugates in Tumor Spheroid Models
Authors: Kirsty McBain, Scientist, Application Development, Sartorius | Last updated: September 2024
Overview
Helping to inform drug discovery and biological research workflows, this poster video highlights how the iQue® High-Throughput Screening (HTS) Cytometry Platform is used with an Incucyte® Live-Cell Analysis System, in a combined workflow, to screen, investigate and compare the anti-tumor activity of antibody-drug conjugates (ADCs) mechanisms of action in vitro, using single and multi-spheroid models.
Traditional monolayer models are cost-effective and scalable but lack many features of the tumor microenvironment, such as complex cell-cell and cell-extracellular matrix interactions. Tumor spheroids afford a more translational model for quantification of ADCs, with more realistic drug effects and a closer relationship to the in vivo scenario.
These data showcase temporal measurements of ADC internalization and cytotoxicity performed using an Incucyte® Live-Cell Analysis System. At assay endpoint, spheroids were dissociated for analysis using the iQue® High-Throughput Screening (HTS) Cytometry Platform to yield further information on spheroid health and composition.
- Document type: Video
- Watch time: 13 minutes
With detailed sections on:
- ADC Internalization
- Multi-Spheroid Cytotoxicity
- Single Spheroid ADC Cytotoxicity
- Bystander Killing
