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Flow cytometry is a powerful technique that identifies defined populations of cells from heterogenous samples such as blood. In a typical experiment, cells are labeled with fluorochrome conjugated antibodies which recognize markers on cells. Labeled single cells are passed through the flow cell where fluorescence is measured by lasers and detectors. Patterns of expression of different markers define the cell phenotype. For example, T-cells are defined by the presence of CD3 antigen within the lymphocyte population. Other markers can be added to identify subpopulations, cell health, activation status, and other parameters.
Secretion of cytokines and other biomarkers can also be used to characterize cells. The iQue® advanced flow cytometer can perform bead-based secreted biomarker detection from the same sample in the same well to provide a complete understanding of the biology of your sample. Beads (microspheres) used for biomarker detection contain different levels of fluorescent dye serving as both identification for the biomarker and a surface for the assay.
Obtain results with very low sample volumes down to 5 µL (in a 384-well plate) conserving precious samples and saving money on reagents.
Whether you are screening thousands of complex samples per day or running a few samples, the iQue® advanced flow cytometer features ease of use, fast walk away operation, and real time data analysis to let you focus on the results of your experiments. With up to 15 parameters and three lasers, this system allows you to gain more information from limited samples.
The iQue® platform includes Forecyt® Software, a complete package that enables every step from acquisition to through data interpretation. Several features of Forecyt® like advanced gating and simple dose-response curve generation standardize and simplify data collection and analysis.
Requiring as little as one microliter of sample with fast and easy acquisition, the iQue® is suitable for a variety of routine and custom assays. Click on the link to see some of our many application examples:
How does it work?
Activation of T-cells leads to several changes including the up-regulation of cell surface markers, cytokine secretion, cell proliferation, and cell death. How can we quickly, objectively and reproducibly quantify this process?
Kits and templated analysis generate data as your cells are analyzed.
Immunophenotyping and Function using iQue® Immune Cell-Based Kits
Screen 152 compounds for their effect on T-cell activation
Example ADCC Assay
Example ADCC Specificity
