Integrated Workflow for Reliable Generation and Selection of 3D iPSC Models

Overview

This case study presents an innovative, automated workflow for developing iPSC-derived 3D models, focusing on monoclonal spheroids and hepatic organoids. Traditional methods for creating disease-specific models are often complex and resource-intensive, here we introduce a streamlined approach utilizing automated selection based on growth and morphology, real-time monitoring, and phenotypic validation. 

Spheroids were successfully generated from single iPSCs using S200 nanowell plates, exhibiting steady growth and high viability. Hepatic organoids cultured in Matrigel^® domes demonstrated proper differentiation and marker expression. This automated system significantly reduces variability and enhances efficiency in drug discovery and toxicity studies, offering broader applications in disease modeling. The study highlights the potential of this approach to simplify the development of physiologically relevant 3D cellular models, advancing research in drug discovery and disease modeling.

Document Type: Case Study

Page Count: 5

Read Time: 10 Minutes

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