Case Study: Monoclonality Assurance with Nanowell Cell Cloning on HEK293SF-3F6 Cell Lines
Last updated: January 2025
Overview
Unlock the potential of HEK293SF-3F6 cell lines with a proven innovative cloning method that not only improves survival rates but accelerates your workflow. The use of 24-well plates, featuring 4,300 nanowells, ensure optimal growth by physically separating cells while allowing essential medium diffusion.
Key Challenges Addressed:
- Monoclonality Validation: Ensuring cell purity for scientific and industrial applications.
- Cross-Contamination Prevention: Utilizing a single capillary during cell transfer to maintain integrity.
- Ghost Well Minimization: Reducing the risk of cell migration into adjacent wells.
With comprehensive image capture every step of the cloning process can be analyzed, from day 0 plating to post-picking analysis. Ghost well reduction techniques ensure implementation of careful handling protocols and maintain a stable incubation environment to minimize disturbances.
Explore how this method from the National Research Council of Canada (NRC) can transform your research and industrial applications today.
- Document type: Case Study
- Page count: 4
- Read time: 5 minutes
- Author: National Research Council of Canada (NRC)
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Key Takeaways
- Improving survival rates and acceleration of workflow
- Optimal growth environment
- Validation of monoclonality
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