Mannose Glycans Content Screening for Human-mAb Samples Using the Octet^® GlyM Kit

The Octet^® GlyM Kit is designed to provide cell line development and upstream bioprocessing groups with a ready to use kit for the rapid relative mannose content screening of both purified and cell harvest antibody samples.

Unlike traditional techniques, the Octet^® platform can also monitor other functional critical quality attributes (CQAs). The ability to view and choose from desired titer and mannose levels at the same time provides more in-depth knowledge that facilitates more informed decisions in the early selection of optimal clones during cell line development.

In this application note, a novel approach for relative mannose content screening and ranking of human monoclonal antibody (mAb) samples is showcased; using a well characterized mAb from the National Institute of Standards and Technology (NIST) as well as samples developed at Novartis.