AAV6 Capsid Optimization with CIM SO3 Plates & Mass Photometry

On-Demand Webinar

This webinar shows new research on AAV6 upstream process optimization for gene therapy manufacturing. The session focuses on strategies to improve full capsid ratio, maintain robust vector titer, increase process understanding, and support more scalable and cost-efficient AAV vector production.  

Sartorius BIA Separations used a Design of Experiments (DoE) approach to systematically evaluate AAV6 production parameters and identify conditions that promote a higher proportion of full capsids while supporting optimal AAV6 vector titer. Further optimization was performed using the Ambr15 system, enabling controlled, small-scale process development with reduced material requirements. CIM^® SO3 Monolithic Well Plates enabled high-throughput purification of upstream samples. Combined with mass photometry, this workflow enables accurate quantification of AAV capsid species, including full, empty, and partially filled particles. By integrating upstream optimization, rapid purification, advanced capsid analytics, and vector titer assessment this approach supports the development of more robust and scalable AAV6 manufacturing workflows for gene therapy applications.