A Simple Method to Determine Relative Potency (EC50) Using Octet® BLI Technology Application Guide
Authors: Stuart Knowling, PhD, Senior Scientist | Last updated: 25/August/2022
Overview
Octet® BLI Discovery and Analysis Software now contains a new module for quickly and easily assessing relative potency using the same assay format currently used for quantitation and kinetic assays, allowing you to measure EC50 similarity using a range of parameter logistic equations (PL), linear and semilog lines. This document introduces basic assay design and an introduction to the new software module.
- Document type: Application Guide
- Page count: 6
- Read time: 10 minutes
Key Takeaways
- New EC50 and IC50 analysis capabilities to determine drug product activities using surrogate potency assays, reducing assay variability, timelines, and costs
- Data bridge to support data transfer between Sartorius Octet® and Sartorius Ambr® bioreactor systems to accelerate cell line development workflows
This Resource is Designed for:
- Biopharma
- CLD
- Academic
- Biotech
- Pharma QC
Applications Supported:
- Biologics
- Lot Release
- Label-free kinetic
- Affinity
- Epitope binning
- Activity
- Concentration
- Potency
- Screening
