Evaluation of Signal Peptides for Enhanced Production Levels In CHO DG44 Cells

The high-titer production of biopharmaceuticals using “Chinese hamster ovary” (CHO) cells is an important pillar of the pharmaceutical industry. Nevertheless, a potential bottleneck for profitable production is the successful secretion of the synthesized proteins through the endoplasmatic reticulum (ER) and the Golgi apparatus into the culture medium directed by the signal peptide (SP). Since natural SP sequences and efficiencies vary between proteins and species, we demonstrated that the utilization of different SPs can lead to enhanced protein expression in already existing production systems.