Integrated 3D Multi-Organ Tox Plate for Real-Time Drug Toxicity Analysis
Document type: Poster | Last updated: August, 2025
Overview
Traditional toxicity screening methods are limited in scope and speed—especially when it comes to capturing complex organ interactions. In this scientific poster, explore how a newly developed Multi-Organ Tox Plate (MOTP) paired with automated live-cell imaging and analysis is redefining how researchers approach in vitro toxicity testing.
What you’ll learn:
- How 3D tissue models representing the intestine, liver, and kidney are integrated into a 96-well plate format
- How real-time toxicity measurements are captured using Cytotox Green Dye and Incucyte® live-cell analysis
- Insights from dose-response experiments using Cisplatin, Fialuridine, and Troglitazone
- Automated EC₅₀ calculations that streamline your workflow
- Why this approach is a scalable, cost-effective alternative to animal models
Key results include:
- Drug-specific organ toxicity trends validated across multiple tissues
- Real-time barrier integrity monitoring via TEER measurements
- High-throughput, reproducible toxicity assessments with reduced manual intervention
Why it matters:
This integrated, tri-organ assay platform enables researchers to evaluate drug safety across multiple organ systems—simultaneously, in real time, and with improved biological relevance.
Download the full poster to explore how this novel workflow accelerates toxicity screening and enhances translational insights.
Key Takeaways
- Multi-organ toxicity tested in parallel: A novel 96-well plate design integrates 3D intestine, liver, and kidney tissue models, enabling simultaneous multi-organ analysis.
- Real-time, automated toxicity assessment: Combines Cytotox Green Dye with Incucyte® live-cell imaging for continuous, hands-off monitoring and automated EC₅₀ calculations.
- High-throughput, scalable workflow: Supports dose-range finding for single or multiple compounds in a streamlined, reproducible format.
- Organ-specific drug responses observed: Fialuridine selectively damaged liver tissue, while Troglitazone impacted both liver and kidney models—highlighting tissue-specific toxicity patterns.
- Comparable data to manual methods: Cisplatin EC₅₀ values matched previously published data, validating the system’s accuracy and reliability.
- Reduces reliance on animal testing: Provides a biologically relevant, economical alternative that enhances predictive toxicology while supporting ethical research practices.
Preview of Poster
