iPSC-Derived Cerebral Organoids: A Tool for Studying Human Neurodevelopment and Pathology
Authors: Sartorius | Last updated: July, 2025
Overview
Traditional neuroscience models fall short in replicating the complexity of the human brain. With low translational success from animal models and limited access to primary brain tissue, researchers are increasingly turning to human pluripotent stem cell (hPSC)-derived brain organoids.
This application note presents a powerful workflow for working with iPSC-derived cerebral organoids — 3D in vitro models that simulate human brain development and disease with region-specific fidelity. Discover how live-cell imaging and high-throughput cytometry come together to enable real-time, non-invasive analysis of organoid health, development, and response to compounds.
- Document type: Application Note
- Page count: 9
Read time: 17 minutes
Key Takeaways
- How to overcome challenges in cerebral organoid analysis
- Strategies for real-time monitoring of growth, morphology, and viability
- Integration of live-cell imaging with high-throughput screening (HTS)
- Quantification of marker expression and cytokine release by cytometry
This Application Note covers:
- The limitations of conventional brain models—and how cerebral organoids address them.
- Challenges in scaling and objectively analyzing 3D organoid cultures.
- A high-throughput, live-cell analysis workflow for monitoring growth, morphology, and viability over time.
- Quantitative methods for assessing marker expression and cytokine release using cytometry.
Whether you’re modeling neurological diseases, testing neuroactive compounds, or developing patient-derived organoids, this resource will help you streamline your workflow and extract more meaningful data—without compromising your models.
