Poster: Automating Robust and Reproducible 3D Culture Model Systems
Last updated: February 2025
Overview
Three-dimensional (3D) models, such as spheroids and organoids, are becoming increasingly important for studying disease and development. Creating these models can be complex, costly, and resource-intensive, requiring efficient and reliable methods for their development, monitoring, and characterization. The complexity increases when producing disease models with cell populations that have specific markers or genetic mutations associated with diseases.
Developing these models necessitates precise cell manipulation to create a physiologically relevant system. In this study, spheroids were cultured from individual induced pluripotent stem cells (iPSCs) to produce clonal iPSC spheroids from a single cell. Human iPSC-derived hepatic organoids were selected from Matrigel® domes based on their attributes and then transferred for further culture and analysis.
We present a simple, standardized, and robust workflow using CellCelector Flex to identify and select 3D cell models for further development based on key growth and morphological attributes. Throughout the workflow, the Incucyte® Live-Cell Analysis System and the iQue® HTS Cytometer were employed to monitor growth and morphological phenotype over time and to perform phenotypic characterization through marker expression analysis.
This approach streamlines the culture and generation of organoids and monoclonal spheroids from iPSCs, facilitating drug discovery, development, and toxicity studies.
- Document type: Poster
- Page count: 1 page
- Read time: 4 minutes
Key Takeaways
- Monoclonal iPSC spheroid generation
- Picking and embedding iPSC spheroids
- Automated identification and isolation of organoids
- Monitoring and characterizing organoid development
Poster Preview
3D models such as spheroids and organoids are becoming increasingly relevant for the study of disease and development.