Application Note: Advanced Synthetic Hydrogel Maintains Pluripotency and Growth in iPSCs
Authors: Daryl Cole, Natasha Lewis, Nicola Bevan, and Kalpana Barnes | Last updated: January 2025
Overview
This application note explores the use of *NexaGel® STEM, a synthetic hydrogel platform, for culturing induced pluripotent stem cells (iPSCs) in both 2D and 3D environments. iPSCs are crucial for applications like disease modeling, regenerative medicine, and drug discovery due to their pluripotency and patient-specific nature. However, traditional 2D cultures on vitronectin are not scalable and may not be suitable for all applications.
NexaGel® provides a defined, xeno-free, and adjustable system that enhances iPSC culture by mimicking the extracellular matrix, ensuring consistency, and minimizing contamination risks. This study shows that iPSCs cultured in NexaGel® retain their pluripotency and viability, with 3D cultures forming spheroid structures that promote cell proliferation. NexaGel® emerges as a promising tool for optimizing iPSC culture and advancing research in both basic and clinical applications.
Document Type: Application Note
Page Count: 8 Pages
Read Time: 11 Minutes
Key Takeaways
- Learn the concept of integrated weighing and the different components involved
- Gain valuable guidance in how to select the correct weighing solution for your application
- Understand the important considerations required for successful installation and what to avoid
This Resource is Designed for:
- OEMs (Original Equipment Manufacturers)
- System Integrators
- Automation Engineers
- Manufacturers in Industries Requiring Precise Weight Measurement
- Process and Production Engineers
- Project Managers
- Maintenance and Calibration Technicians
- Chemical and Pharmaceutical Industries
Applications Supported:
- Powder Dosing
- Thermogravimetric Analysis
- Real-time weight-based process control
- Medical Device Manufacturing
Application Note
Figure 1. Advanced Synthetic Hydrogel Maintains Pluripotency and Growth in iPSCs.
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Add at bottom of page in small text: *NexaGel® is a registered trademark of Sartorius Bioanalytical Instruments, Inc. For details on registrations, please refer to our website https://www.sartorius.com/en/patents-and-trademarks.