Purification of Extracellular Vesicles from MSCs Grown in 3D Culture

Robust, scalable, and well-characterized production methods are essential to realize the full clinical potential of extracellular vesicles (EVs).
This application note presents an end-to-end process for the production and purification of EVs from mesenchymal stromal cells (MSCs). The established method addresses challenges related to scalability and reliable analytics, providing a robust foundation for clinical-scale production. This comprehensive approach ensures the production of high-quality EVs, paving the way for their application in therapeutic and diagnostic fields.

Key Learning Objectives

1. Explore culture parameters that support a robust upstream EV production process
2. Discover a downstream workflow that maximizes EV retention while reducing contaminants
3. See how orthogonal analytics confirm EV purity, identity, and functional activity