On-Demand Webinar - Streamlining Hepatic Differentiation: Using iPSCs for Organoid Production
Webinar duration: 27 minutes
Overview
The effectiveness of stem cells in clinical and laboratory research, has been clearly demonstrated through studies illustrating them as models for many diseases and therapeutic developments. The fundamental flexibility of stem cells allows for the creation of many different tissue groups, originating from a single, highly proliferative base cell type. Differentiation of induced pluripotent stem cells (iPSCs) into somatic tissues requires specific conditions depending on the desired tissue group.
This webinar describes work performed to differentiate human iPSCs into hepatocytes, using three distinct media types with their own growth factor and cytokine supplementation, at key stages of differentiation. Hence providing a relatively simple method for producing mature hepatic cells. This has enabled the production of human hepatic organoids from these source cells, demonstrating a robust method for unlimited organoid development.
Outlined is a simple, standardized and robust workflow, using Research Use Only (RUO) Growth Factors and Cytokines, in combination with high-throughput screening cytometry for phenotypic characterization, and live-cell analysis for monitoring cell growth.
Drug discovery, development and toxicity studies, each benefit from this simplified approach to the generation of functional hepatic cells and organoids from iPSCs.
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This Webinar Includes:
- Differentiation details of iPSCs into hepatic lineage cells and organoids
- Information on the dissociation of complex 3D structures into healthy single cells for analysis
Tips on the development of robust protocols for organoid production
Webinar Speaker
Daryl Cole Ph.D.
Scientist, Sartorius
Daryl Cole earned a Ph.D. in Molecular Oncology from King’s College London in 2008 and joined Sartorius in 2021 as a Scientist in the Bio Applications Team, working with the Incucyte®, Octet®, iQue®, and CellCelector.
He has over 15 years of experience in a range of scientific fields from cancer research to organoid and stem cell development.
