Delivering robust, reproducible solutions to complex tissue model culture
Authors: Daryl Cole and Nicola Bevan | Last updated: May 2025
Overview
This poster outlines an innovative automated workflow designed for the development and monitoring of 3D cell culture models, specifically targeting induced pluripotent stem cell (iPSC) spheroids and hepatic organoids. By employing the CellCelector Flex platform, researchers can efficiently select and embed these models based on specific growth and morphological attributes.
The Incucyte® Live-Cell Analysis System is utilized to monitor growth and morphological changes over time, while the iQue® HTS Cytometer offers phenotypic characterization through marker expression analysis. This process facilitates the generation of monoclonal iPSC spheroids from single GFP-expressing cells and the isolation of organoids from Matrigel® domes, both essential for drug discovery and toxicology studies.
The results demonstrate that the picked spheroids and organoids maintain viability and expression profiles, underscoring the effectiveness of this standardized approach in creating physiologically relevant tissue models, thereby enhancing research reliability and reproducibility.
Document Type: Poster
Page Count: 1
Read Time: 5 Minutes
Key Takeaways
- Automated workflow efficiency
- Phenotypic characterization
- Enhanced reliability and reproducibility
- Monoclonal iPSC spheroid generation
